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Western Blot (WB) (Western blot analysis of ZIMP10 in K562 cell lysate with ZIMP10 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide)

Rabbit anti-Human ZIMP10 Polyclonal Antibody | anti-ZMIZ1 antibody

ZIMP10 Antibody

Gene Names
ZMIZ1; MIZ; RAI17; ZIMP10; hZIMP10; TRAFIP10
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
ZIMP10 Antibody is affinity chromatography purified via peptide column.
Synonyms
ZIMP10; Polyclonal Antibody; ZIMP10 Antibody; MIZ; RAI17; hZIMP10; TRAFIP10; KIAA1224; Zinc finger MIZ domain-containing protein 1; PIAS-like protein Zimp10; zinc finger; MIZ-type containing 1; anti-ZMIZ1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
At least three isoforms are known to exist; this antibody will recognize all three. ZIMP10 antibody is predicted to not cross-react with other PIAS protein family members.
Purity/Purification
ZIMP10 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
1067
Applicable Applications for anti-ZMIZ1 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ZIMP10 antibody can be used for detection of ZIMP10 by Western blot at 0.5 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
ZIMP10 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ZIMP10.
Buffer
ZIMP10 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
ZIMP10 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of ZIMP10 in K562 cell lysate with ZIMP10 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide)

Western Blot (WB) (Western blot analysis of ZIMP10 in K562 cell lysate with ZIMP10 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide)

Immunocytochemistry (IHC)

(Immunocytochemistry of ZIMP10 in K562 cells with ZIMP10 antibody at 10 μg/mL.)

Immunocytochemistry (IHC) (Immunocytochemistry of ZIMP10 in K562 cells with ZIMP10 antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of ZIMP10 in K562 cells with ZIMP10 antibody at 20 μg/mL.)

Immunofluorescence (IF) (Immunofluorescence of ZIMP10 in K562 cells with ZIMP10 antibody at 20 μg/mL.)
Related Product Information for anti-ZMIZ1 antibody
ZIMP10 Antibody: ZIMP10, also known as ZMIZ1, is a novel PIAS (protein inhibitor of activated signal transducer and activator of transcription)-like protein initially identified as a transcriptional co-activator of the androgen receptor (AR). ZIMP10 and the related protein ZIMP7 interact with PIAS3 and enhances AR-mediated transcription. Later experiments showed that ZIMP10 is also a co-activator of the p53 tumor suppressor. Mice deficient in ZIMP10 result in embryonic lethality by E10.5; these embryos reveal severe defects in the reorganization of the yolk sac vascular plexus, indicating that ZIMP10 plays an important role in proper vascular development.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
107,270 Da
NCBI Official Full Name
zinc finger MIZ domain-containing protein 1
NCBI Official Synonym Full Names
zinc finger, MIZ-type containing 1
NCBI Official Symbol
ZMIZ1
NCBI Official Synonym Symbols
MIZ; RAI17; ZIMP10; hZIMP10; TRAFIP10
NCBI Protein Information
zinc finger MIZ domain-containing protein 1; RP11-519K18.1; FLJ00092 protein; retinoic acid induced 17; zinc finger-containing, Miz1, PIAS-like protein on chromosome 10
UniProt Protein Name
Zinc finger MIZ domain-containing protein 1
UniProt Gene Name
ZMIZ1
UniProt Synonym Gene Names
KIAA1224; RAI17; ZIMP10
UniProt Entry Name
ZMIZ1_HUMAN

NCBI Description

This gene encodes a member of the PIAS (protein inhibitor of activated STAT) family of proteins. The encoded protein regulates the activity of various transcription factors, including the androgen receptor, Smad3/4, and p53. The encoded protein may also play a role in sumoylation. A translocation between this locus on chromosome 10 and the protein tyrosine kinase ABL1 locus on chromosome 9 has been associated with acute lymphoblastic leukemia. [provided by RefSeq, Mar 2010]

Uniprot Description

RAI17: Increases ligand-dependent transcriptional activity of AR and promotes AR sumoylation. The stimulation of AR activity is dependent upon sumoylation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 10q22.3

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; nuclear speck

Molecular Function: zinc ion binding

Biological Process: heart morphogenesis; developmental growth; positive regulation of fibroblast proliferation; transcription, DNA-dependent; in utero embryonic development; vitellogenesis; artery morphogenesis; cell aging; positive regulation of transcription from RNA polymerase II promoter; vasculogenesis

Research Articles on ZMIZ1

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Product Notes

The ZMIZ1 zmiz1 (Catalog #AAA151128) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZIMP10 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZIMP10 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). ZIMP10 antibody can be used for detection of ZIMP10 by Western blot at 0.5 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the ZMIZ1 zmiz1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZIMP10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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