Granzyme B Antibody | anti-GZMB antibody

Granzyme B Antibody

Cat. Num. AAA853854

• Gene Names: GZMB; C11; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: Granzyme B; Granzyme B Antibody; 3.4.21.79; CCPI; CGL-1; CGL1; CSP-B; CSPB; CTLA1; CTSGL1; HLP; SECT; PROTEASE SERINE B; T-cell serine protease 1-3E; cathepsin G-like 1; cytotoxic serine protease B; fragmentin 2; granzyme B (granzyme 2; cytotoxic T-lymphocyte-associated serine esterase 1); granzyme B precursor; anti-GZMB antibody

• Reactivity: Human, Mouse, Rat
• Specificity: Granzyme B antibody detects endogenous levels of total Granzyme B protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 247

• Applicable Applications for anti-GZMB antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:5000
• Immunogen: The antiserum was produced against synthesized peptide derived from human Granzyme B.
• Preparation and Storage: Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Granzyme B antibody.)

Western Blot (WB)

(Western blot analysis of extracts from NIH/3T3 cells, using Granzyme B antibody.)

References

David A. Jans, J. Biol. Chem., Nov 1996; 271: 30781. Eugene Estella, Diabetes, Aug 2006; 55: 2212-2219. Yong Gu, J. Biol. Chem., May 1996; 271: 10816. Jiuru Sun, J. Biol. Chem., Nov 1996; 271: 27802.

NCBI and Uniprot Product Information

• NCBI GI #: 221625528
• NCBI GeneID: 3002
• NCBI Accession #: NP_004122.2
• NCBI GenBank Nucleotide #: NM_004131.5
• UniProt Accession #: P10144; Q8N1D2; Q9UCC1
• Molecular Weight: 27,716 Da
• NCBI Official Full Name: granzyme B isoform 1 preproprotein
• NCBI Official Synonym Full Names: granzyme B
• NCBI Official Symbol: GZMB
• NCBI Official Synonym Symbols: C11; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
• NCBI Protein Information: granzyme B
• UniProt Protein Name: Granzyme B
• Protein Family: Granzyme
• UniProt Gene Name: GZMB
• UniProt Synonym Gene Names: CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP

NCBI Description

This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016]

Uniprot Description

This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.