WNT10B sirna

WNT10B siRNA (Human)

Cat. Num. AAA8224501

• Gene Names: WNT10B; SHFM6; WNT-12
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WNT10B; WNT10B siRNA (Human); WNT12; Protein Wnt-10b; Protein Wnt-12; WNT10B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: WNT10B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 389

• Applicable Applications for WNT10B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human WNT10B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WNT10B sirna

siRNA to inhibit WNT10B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16936522
• NCBI GeneID: 7480
• NCBI Accession #: NP_003385.2
• NCBI GenBank Nucleotide #: NM_003394.3
• UniProt Accession #: O00744; O00747; Q4VAJ4; Q4VAJ5; Q8WZ97; B2R7A5
• Molecular Weight: 20,483 Da
• NCBI Official Full Name: protein Wnt-10b
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family, member 10B
• NCBI Official Symbol: WNT10B
• NCBI Official Synonym Symbols: SHFM6; WNT-12
• NCBI Protein Information: protein Wnt-10b
• UniProt Protein Name: Protein Wnt-10b
• UniProt Gene Name: WNT10B
• UniProt Synonym Gene Names: WNT12
• UniProt Entry Name: WN10B_HUMAN

NCBI Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region. [provided by RefSeq, Jul 2008]

Uniprot Description

WNT10B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Defects in WNT10B are the cause of split-hand/foot malformation type 6 (SHFM6). SHFM is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. SHFM6 is a autosomal recessive disorder. Belongs to the Wnt family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: frizzled binding; receptor agonist activity

Biological Process: positive regulation of apoptosis; skeletal muscle fiber development; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; neuron differentiation; positive regulation of cell proliferation; chondrocyte differentiation; positive regulation of epithelial cell differentiation; cell cycle arrest; G2/M transition of mitotic cell cycle; positive regulation of transcriptional preinitiation complex assembly; negative regulation of epithelial cell proliferation; smoothened signaling pathway; cell fate commitment; Wnt receptor signaling pathway; sensory perception of taste; protein stabilization; regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of fat cell differentiation; positive regulation of bone mineralization; positive regulation of osteoblast differentiation; positive regulation of transcription factor activity; regulation of skeletal muscle development; lipid metabolic process; positive regulation of anagen; myoblast cell differentiation involved in skeletal muscle regeneration

Disease: Split-hand/foot Malformation 6