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Western Blot (WB) (All lanes : Anti-RPS4X Antibody at 1:1000 dilutionLane 1: Hela whole cell lysatesLane 2: A431 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 30 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit RPS4X Polyclonal Antibody | anti-RPS4X antibody

RPS4X Antibody

Gene Names
RPS4X; S4; CCG2; RPS4; SCAR; SCR10; DXS306
Reactivity
Human, mouse, rat
Applications
Western Blot, ELISA, Immunocytochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RPS4X; Polyclonal Antibody; RPS4X Antibody; 40S ribosomal protein S4; X isoform; SCR10; Single copy abundant mRNA protein; CCG2; RPS4; SCAR; anti-RPS4X antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse, rat
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%
Concentration
1mg/ml (varies by lot)
Sequence Positions
71-130
Sequence Length
263
Applicable Applications for anti-RPS4X antibody
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
Application Notes
WB~~ 1:1000
Antigen Type
synthetic peptide
Antigen Source
KLH conjugated synthetic peptide derived from human RPS4X
Preparation and Storage
Store at -20 degree C.Stable for 12 months from date of receipt

Western Blot (WB)

(All lanes : Anti-RPS4X Antibody at 1:1000 dilutionLane 1: Hela whole cell lysatesLane 2: A431 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 30 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-RPS4X Antibody at 1:1000 dilutionLane 1: Hela whole cell lysatesLane 2: A431 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 30 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
References
Fisher E.M.C.,et al.Cell 63:1205-1218(1990).
Watanabe M.,et al.J. Cell Sci. 100:35-43(1991).
Zuo L.,et al.Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Dmitrenko V.V.,et al.Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29598
NCBI Official Full Name
40S ribosomal protein S4, X isoform X isoform
NCBI Official Synonym Full Names
ribosomal protein S4, X-linked
NCBI Official Symbol
RPS4X
NCBI Official Synonym Symbols
S4; CCG2; RPS4; SCAR; SCR10; DXS306
NCBI Protein Information
40S ribosomal protein S4, X isoform
UniProt Protein Name
40S ribosomal protein S4, X isoform
Protein Family
UniProt Gene Name
RPS4X
UniProt Synonym Gene Names
CCG2; RPS4; SCAR
UniProt Entry Name
RS4X_HUMAN

NCBI Description

Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes ribosomal protein S4, a component of the 40S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene and ribosomal protein S4, Y-linked (RPS4Y). The 2 isoforms encoded by these genes are not identical, but are functionally equivalent. Ribosomal protein S4 belongs to the S4E family of ribosomal proteins. This gene is not subject to X-inactivation. It has been suggested that haploinsufficiency of the ribosomal protein S4 genes plays a role in Turner syndrome; however, this hypothesis is controversial. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPS4X: Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes ribosomal protein S4, a component of the 40S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene and ribosomal protein S4, Y-linked (RPS4Y). The 2 isoforms encoded by these genes are not identical, but are functionally equivalent. Ribosomal protein S4 belongs to the S4E family of ribosomal proteins. This gene is not subject to X-inactivation. It has been suggested that haploinsufficiency of the ribosomal protein S4 genes plays a role in Turner syndrome; however, this hypothesis is controversial. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Protein type: Ribosomal; RNA-binding; Translation

Chromosomal Location of Human Ortholog: Xq13.1

Cellular Component: polysome; small ribosomal subunit; focal adhesion; membrane; ribosome; cytosol; ribonucleoprotein complex

Molecular Function: rRNA binding; structural constituent of ribosome

Biological Process: SRP-dependent cotranslational protein targeting to membrane; translation; positive regulation of translation; viral reproduction; multicellular organismal development; viral infectious cycle; translational termination; translational elongation; cellular protein metabolic process; positive regulation of cell proliferation; mRNA catabolic process, nonsense-mediated decay; translational initiation; gene expression; viral transcription

Research Articles on RPS4X

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Product Notes

The RPS4X rps4x (Catalog #AAA9209831) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 71-130. The RPS4X Antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's RPS4X can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC). WB~~ 1:1000. Researchers should empirically determine the suitability of the RPS4X rps4x for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPS4X, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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