TRIM27 sirna

TRIM27 siRNA (Human)

Cat. Num. AAA8221198

• Gene Names: TRIM27; RFP; RNF76
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TRIM27; TRIM27 siRNA (Human); RFP; RNF76; Zinc finger protein RFP; RING finger protein 76; Ret finger protein; Tripartite motif-containing protein 27; TRIM27 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TRIM27 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 358

• Applicable Applications for TRIM27 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TRIM27 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TRIM27 sirna

siRNA to inhibit TRIM27 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 5730009
• NCBI GeneID: 5987
• NCBI Accession #: NP_006501.1
• NCBI GenBank Nucleotide #: NM_006510.4
• UniProt Accession #: P14373; Q5RJA8; Q5ST26; Q6LA73; Q6NXR9; Q9BZY6; Q9UJL3; A2BE15
• Molecular Weight: 41,439 Da
• NCBI Official Full Name: zinc finger protein RFP
• NCBI Official Synonym Full Names: tripartite motif containing 27
• NCBI Official Symbol: TRIM27
• NCBI Official Synonym Symbols: RFP; RNF76
• NCBI Protein Information: zinc finger protein RFP
• UniProt Protein Name: Zinc finger protein RFP
• Protein Family: Zinc finger protein
• UniProt Gene Name: TRIM27
• UniProt Synonym Gene Names: RFP; RNF76
• UniProt Entry Name: TRI27_HUMAN

NCBI Description

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. [provided by RefSeq, Jul 2008]

Uniprot Description

TRIM27: Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA. E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation. Defects in TRIM27 are a cause of thyroid papillary carcinoma (TPC). TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. A chromosomal aberration involving TRIM27/RFP is found in thyroid papillary carcinomas. Translocation t(6;10)(p21.3;q11.2) with RET. The translocation generates TRIM27/RET and delta TRIM27/RET oncogenes. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; EC 6.3.2.-; Oncoprotein; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 6p22

Cellular Component: nucleoplasm; PML body; nuclear membrane; membrane; integral to plasma membrane; retromer complex; cytoplasm; early endosome; nucleus; endosome

Molecular Function: protein binding; DNA binding; nucleic acid binding; zinc ion binding; metal ion binding; ubiquitin-protein ligase activity; transmembrane receptor protein tyrosine kinase activity; ligase activity

Biological Process: cell proliferation; negative regulation of viral transcription; peptidyl-tyrosine phosphorylation; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; negative regulation of adaptive immune response; negative regulation of protein kinase activity; innate immune response; positive regulation of transcription factor activity; spermatogenesis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; retrograde transport, endosome to Golgi