TARBP2 sirna

TARBP2 siRNA (Human)

Cat. Num. AAA8231052

• Gene Names: TARBP2; LOQS; TRBP; TRBP1; TRBP2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TARBP2; TARBP2 siRNA (Human); TRBP; RISC-loading complex subunit TARBP2; TAR RNA-binding protein 2; Trans-activation-responsive RNA-binding protein; TARBP2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TARBP2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 345

• Applicable Applications for TARBP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TARBP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TARBP2 sirna

siRNA to inhibit TARBP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 19743838
• NCBI GeneID: 6895
• NCBI Accession #: NP_004169.3
• NCBI GenBank Nucleotide #: NM_004178.4
• UniProt Accession #: Q15633; Q12878; Q8WY32; Q8WY33; Q9BRY2
• Molecular Weight: 36,916 Da
• NCBI Official Full Name: RISC-loading complex subunit TARBP2 isoform b
• NCBI Official Synonym Full Names: TAR (HIV-1) RNA binding protein 2
• NCBI Official Symbol: TARBP2
• NCBI Official Synonym Symbols: LOQS; TRBP; TRBP1; TRBP2
• NCBI Protein Information: RISC-loading complex subunit TARBP2
• UniProt Protein Name: RISC-loading complex subunit TARBP2
• Protein Family: RISC-loading complex
• UniProt Gene Name: TARBP2
• UniProt Entry Name: TRBP2_HUMAN

NCBI Description

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene also has a pseudogene. [provided by RefSeq, Jul 2008]

Uniprot Description

TRBP: the HIV-1 TAR RNA binding protein (TRBP) is a double strand RNA binding protein (dsRBP) that is required for the recruitment of Ago2 to the small interfering RNA (siRNA) bound by Dicer. TRBP increases HIV-1 expression and replication by inhibiting the interferon-induced protein kinase PKR and by increasing translation of viral mRNA. Dicer, Ago2, and TRBP are the main components of the RNA-induced silencing complex (RISC).

Protein type: Motility/polarity/chemotaxis; RNA processing; Translation; RNA-binding

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: nucleoplasm; perinuclear region of cytoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; miRNA binding; protein homodimerization activity; siRNA binding; double-stranded RNA binding

Biological Process: regulation of translation; regulation of transcription from RNA polymerase II promoter; positive regulation of viral genome replication; regulation of transcription, DNA-dependent; pre-microRNA processing; regulation of viral transcription; RNA interference, targeting of mRNA for destruction; miRNA-mediated gene silencing, miRNA loading onto RISC; negative regulation of protein kinase activity; gene expression; negative regulation of defense response to virus by host; RNA interference, production of siRNA