Rabbit anti-Human TRBP Polyclonal Antibody | anti-TRBP antibody

TRBP Polyclonal Antibody

Cat. Num. AAA668164

• Gene Names: TARBP2; LOQS; TRBP; TRBP1; TRBP2
• Reactivity: Human
• Applications: Western Blot
• Synonyms: TRBP; Polyclonal Antibody; TRBP Polyclonal Antibody; anti-TRBP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Form/Format: Purified (Liquid)
• Concentration: 0.5mg/ml, 200µl (varies by lot)
• Sequence Length: 345

• Applicable Applications for anti-TRBP antibody: Western Blot (WB)
• Application Notes: Western Blot: 6-7 µg/ml
• Antigen: TRBP
• Immunogen: A partial recombinant TRBP protein (amino acids 150-366) was used as the immunogen for this antibody.
• Preparation and Storage: Store antibody at 4 degree C, stable for 6 months. For long-term storage, store at -20 degree C. Avoid repeated freeze and thaw cycles. Expire 1 year from date of purchase.

Western Blot (WB)

( Expression analysis of TRBP. Anti-TRBP antibody (11-13003) was used at 6 µg/ml on Hela lysate.)

NCBI and Uniprot Product Information

• NCBI GI #: 19743838
• NCBI GeneID: 6895
• NCBI Accession #: NP_004169.3
• NCBI GenBank Nucleotide #: NM_004178.4
• UniProt Accession #: Q15633; Q12878; Q8WY32; Q8WY33; Q9BRY2
• Molecular Weight: 36,916 Da
• NCBI Official Full Name: RISC-loading complex subunit TARBP2 isoform b
• NCBI Official Synonym Full Names: TARBP2, RISC loading complex RNA binding subunit
• NCBI Official Symbol: TARBP2
• NCBI Official Synonym Symbols: LOQS; TRBP; TRBP1; TRBP2
• NCBI Protein Information: RISC-loading complex subunit TARBP2
• UniProt Protein Name: RISC-loading complex subunit TARBP2
• UniProt Gene Name: TARBP2
• UniProt Entry Name: TRBP2_HUMAN

NCBI Description

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene also has a pseudogene. [provided by RefSeq, Jul 2008]

Uniprot Description

TRBP: the HIV-1 TAR RNA binding protein (TRBP) is a double strand RNA binding protein (dsRBP) that is required for the recruitment of Ago2 to the small interfering RNA (siRNA) bound by Dicer. TRBP increases HIV-1 expression and replication by inhibiting the interferon-induced protein kinase PKR and by increasing translation of viral mRNA. Dicer, Ago2, and TRBP are the main components of the RNA-induced silencing complex (RISC).

Protein type: RNA-binding; Translation; RNA processing; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: nucleoplasm; perinuclear region of cytoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; miRNA binding; siRNA binding; protein homodimerization activity; double-stranded RNA binding

Biological Process: regulation of translation; regulation of transcription from RNA polymerase II promoter; positive regulation of viral genome replication; regulation of transcription, DNA-dependent; regulation of viral transcription; pre-microRNA processing; RNA interference, targeting of mRNA for destruction; miRNA-mediated gene silencing, miRNA loading onto RISC; negative regulation of protein kinase activity; gene expression; negative regulation of defense response to virus by host; RNA interference, production of siRNA