TARBP2 recombinant protein

TARBP2, 1-345aa, Human, E Coli (Denatured)

Cat. Num. AAA205533

• Gene Names: TARBP2; LOQS; TRBP; TRBP1; TRBP2
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: TARBP2; 1-345aa; Human; E Coli (Denatured); TAR RNA binding protein 2 isoform b; LOQS; TRBP; TRBP1; TRBP2; TARBP2 recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol 0.4M urea
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MLAANPGKTP ISLLQEYGTR IGKTPVYDLL KAEGQAHQPN FTFRVTVGDT SCTGQGPSKK AAKHKAAEVA LKHLKGGSML EPALEDSSSF SPLDSSLPED IPVFTAAAAA TPVPSVVLTR SPPMELQPPV SPQQSECNPV GALQELVVQK GWRLPEYTVT QESGPAHRKE FTMTCRVERF IEIGSGTSKK LAKRNAAAKM LLRVHTVPLD ARDGNEVEPD DDHFSIGVGS RLDGLRNRGP GCTWDSLRNS VGEKILSLRS CSLGSLGALG PACCRVLSEL SEEQAFHVSY LDIEELSLSG LCQCLVELST QPATVCHGSA TTREAARGEA ARRALQYLKI MAGSK
• Sequence Length: 345

• Applicable Applications for TARBP2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for TARBP2 recombinant protein

TARBP2 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. This protein contains 3 DRBM (double-stranded RNA-binding) domains. It may also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. TARBP2 binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. Recombinant human TARBP2 protein was expressed in E Coli.

Product Categories/Family for TARBP2 recombinant protein

Epigenomics (Transcription & Translation)

References

Dorin D., et al (2003). J. Biol. Chem. 278:4440-4448; Gregory R.I., et al (2005). Cell 123:631-640;

NCBI and Uniprot Product Information

• NCBI GI #: 19743838
• NCBI GeneID: 6895
• NCBI Accession #: NP_004169
• NCBI GenBank Nucleotide #: NM_004178.4
• UniProt Accession #: Q15633; Q12878; Q8WY32; Q8WY33; Q9BRY2
• Molecular Weight: 36.9 kDa (345aa)
• NCBI Official Full Name: RISC-loading complex subunit TARBP2 isoform b
• NCBI Official Synonym Full Names: TARBP2, RISC loading complex RNA binding subunit
• NCBI Official Symbol: TARBP2
• NCBI Official Synonym Symbols: LOQS; TRBP; TRBP1; TRBP2
• NCBI Protein Information: RISC-loading complex subunit TARBP2
• UniProt Protein Name: RISC-loading complex subunit TARBP2
• Protein Family: RISC-loading complex
• UniProt Gene Name: TARBP2

NCBI Description

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene also has a pseudogene. [provided by RefSeq, Jul 2008]

Uniprot Description

Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha. May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR.