Canine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit | TIMP2 elisa kit

Canine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit

Cat. Num. AAA451777

• Reactivity: Canine
• Synonyms: Tissue Inhibitors Of Metalloproteinase 2 (TIMP2); Canine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit; CSC-21K; TIMP Metallopeptidase Inhibitor 2; TIMP2 elisa kit

• Reactivity: Canine
• Specificity: This assay has high sensitivity and excellent specificity for detection of TIMP2. No significant cross-reactivity or interference between TIMP2 and analogues was observed.
• Sequence Length: 220

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 6.25-400ng/mL
• Sensitivity: 1.5ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TIMP2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TIMP2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TIMP2 elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TIMP2 in canine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TIMP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TIMP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TIMP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TIMP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 50978760
• NCBI GeneID: 403633
• NCBI Accession #: NP_001003082.1
• NCBI GenBank Nucleotide #: NM_001003082.1
• UniProt Accession #: Q9TTY1; O77804; Q9TTE9
• Molecular Weight: 24,297 Da
• NCBI Official Full Name: metalloproteinase inhibitor 2
• NCBI Official Symbol: TIMP2
• NCBI Protein Information: metalloproteinase inhibitor 2
• UniProt Protein Name: Metalloproteinase inhibitor 2
• Protein Family: Metalloproteinase inhibitor
• UniProt Gene Name: TIMP2
• UniProt Synonym Gene Names: TIMP-2

Uniprot Description

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.