SPANXB1 sirna

SPANXB1 siRNA (Human)

Cat. Num. AAA8203883

• Gene Names: SPANXB1; B1; CT11.2; SPANXB; SPANX-B; SPANXB2; SPANXF1; SPANXF2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SPANXB1; SPANXB1 siRNA (Human); SPANXB; Sperm protein associated with the nucleus on the X chromosome B/F; Cancer/testis antigen 11.2; CT11.2; Nuclear-associated protein SPAN-Xb; SPANX-B; Nuclear-associated protein SPAN-Xf; SPANX-F; SPANX family member B/F; SPANXB1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SPANXB1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 103

• Applicable Applications for SPANXB1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SPANXB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SPANXB1 sirna

siRNA to inhibit SPANXB1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 608601818
• NCBI GeneID: 728695
• NCBI Accession #: NP_115850.2
• NCBI GenBank Nucleotide #: NM_032461.3
• UniProt Accession #: Q9NS25; Q32WQ7; Q5JYZ7; Q8TAD5; B2RPP2
• Molecular Weight: 11,840 Da
• NCBI Official Full Name: sperm protein associated with the nucleus on the X chromosome B1
• NCBI Official Synonym Full Names: SPANX family, member B1
• NCBI Official Symbol: SPANXB1
• NCBI Official Synonym Symbols: B1; CT11.2; SPANXB; SPANX-B; SPANXB2; SPANXF1; SPANXF2
• NCBI Protein Information: sperm protein associated with the nucleus on the X chromosome B1
• UniProt Protein Name: Sperm protein associated with the nucleus on the X chromosome B1
• Protein Family: Sperm protein associated with the nucleus on the X chromosome
• UniProt Gene Name: SPANXB1
• UniProt Synonym Gene Names: SPANXB; SPANXB2; SPANXF1; SPANX-B
• UniProt Entry Name: SPNXB_HUMAN

NCBI Description

Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family of cancer/testis-associated genes, which are located in a cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular family member contains an additional 18 nucleotides in its coding region compared to the other family members in the same gene cluster. This family member is also subject to gene copy number variation. Although the protein encoded by this gene contains consensus nuclear localization signals, the major site for subcellular localization of expressed protein is in the cytoplasmic droplets of ejaculated spermatozoa. This protein provides a biochemical marker for studying the unique structures in spermatazoa, while attempting to further define its role in spermatogenesis. [provided by RefSeq, Apr 2014]

Uniprot Description

SPANXB1: Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family of cancer/testis-associated genes, which are located in a cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular gene maps to chromosome X in a head-to-tail orientation with SPANX family member B2, which appears to be a duplication of the B1 locus. The SPANXB genes are unique members of this gene family, since they contain an additional 18 nt in their coding region compared to the majority of family members. Although the protein encoded by this gene contains consensus nuclear localization signals, the major site for subcellular localization of expressed protein is in the cytoplasmic droplets of ejaculated spermatozoa. This protein provides a biochemical marker for studying the unique structures in spermatazoa, while attempting to further define its role in spermatogenesis. [provided by RefSeq, Jul 2008]

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Xq27.1

Cellular Component: cytoplasm; nucleus

Biological Process: spermatid development