Rabbit anti-Human SPNXB Polyclonal Antibody | anti-SPNXB antibody

SPNXB, ID (SPANXB1, SPANXB, Sperm protein associated with the nucleus on the X chromosome B/F, Cancer/testis antigen 11.2, Nuclear-associated protein SPAN-Xb, Nuclear-associated protein SPAN-Xf, SPANX family member B/F) (Azide free) (HRP)

Cat. Num. AAA6350699

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SPNXB; Polyclonal Antibody; ID (SPANXB1; SPANXB; Sperm protein associated with the nucleus on the X chromosome B/F; Cancer/testis antigen 11.2; Nuclear-associated protein SPAN-Xb; Nuclear-associated protein SPAN-Xf; SPANX family member B/F) (Azide free) (HRP); SPANXB1; SPANX family member B/F; anti-SPNXB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SPNXB antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SPNXB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-47 amino acids from the Central region of human SPNXB.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SPNXB Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the SPNXB antibody detected the SPNXB protein (arrow).)

Related Product Information for anti-SPNXB antibody

Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family of cancer/testis-associated genes, which are located in a cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular gene maps to chromosome X in a head-to-tail orientation with SPANX family member B1 and appears to be a duplication of that locus. The SPANXB genes are unique members of this gene family, since they contain an additional 18 nt in their coding region compared to the majority of family members. Although the protein encoded by this gene contains consensus nuclear localization signals, the major site for subcellular localization of expressed protein is in the cytoplasmic droplets of ejaculated spermatozoa. This protein provides a biochemical marker for studying the unique structures in spermatazoa, while attempting to further define its role in spermatogenesis.

Product Categories/Family for anti-SPNXB antibody

Antibodies; Cancer Markers

References

Hansen, S., et al. Syst Biol Reprod Med 55, 18-26 (2010): Hansen, M.A., et al. Mol. Reprod. Dev. 75(2):219-229(2008). Kouprina, N., et al. Genome Res. 15(11):1477-1486(2005). Ross, M.T., et al. Nature 434(7031):325-337(2005). Zendman, A.J., et al. Gene 309(2):125-133(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 22027492
• NCBI GeneID: 100133171
• NCBI Accession #: NP_663697.1
• NCBI GenBank Nucleotide #: NM_145664.1
• UniProt Accession #: Q9NS25; Q32WQ7; Q5JYZ7; Q8TAD5; A0A0G2JJ56; B2RPP2
• Molecular Weight: 11,840 Da
• UniProt Protein Name: Sperm protein associated with the nucleus on the X chromosome B/F
• UniProt Gene Name: SPANXB1
• UniProt Synonym Gene Names: SPANXB; CT11.2; SPANX-B; SPANX-F
• UniProt Entry Name: SPNXB_HUMAN

Uniprot Description

SPANXB1: Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family of cancer/testis-associated genes, which are located in a cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular gene maps to chromosome X in a head-to-tail orientation with SPANX family member B2, which appears to be a duplication of the B1 locus. The SPANXB genes are unique members of this gene family, since they contain an additional 18 nt in their coding region compared to the majority of family members. Although the protein encoded by this gene contains consensus nuclear localization signals, the major site for subcellular localization of expressed protein is in the cytoplasmic droplets of ejaculated spermatozoa. This protein provides a biochemical marker for studying the unique structures in spermatazoa, while attempting to further define its role in spermatogenesis. [provided by RefSeq, Jul 2008]

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Xq27.1

Cellular Component: cytoplasm; nucleus

Biological Process: spermatid development