SIPA1 sirna

SIPA1 siRNA (Human)

Cat. Num. AAA8219458

• Gene Names: SIPA1; SPA1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SIPA1; SIPA1 siRNA (Human); SPA1; Signal-induced proliferation-associated protein 1; Sipa-1; GTPase-activating protein Spa-1; p130 SPA-1; SIPA1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SIPA1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1042

• Applicable Applications for SIPA1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SIPA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SIPA1 sirna

siRNA to inhibit SIPA1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 206725523
• NCBI GeneID: 6494
• NCBI Accession #: NP_006738.3
• NCBI GenBank Nucleotide #: NM_006747.3
• UniProt Accession #: Q96FS4; O14518; O60484; O60618; Q2YD83
• Molecular Weight: 112,149 Da
• NCBI Official Full Name: signal-induced proliferation-associated protein 1
• NCBI Official Synonym Full Names: signal-induced proliferation-associated 1
• NCBI Official Symbol: SIPA1
• NCBI Official Synonym Symbols: SPA1
• NCBI Protein Information: signal-induced proliferation-associated protein 1
• UniProt Protein Name: Signal-induced proliferation-associated protein 1
• Protein Family: Signal-induced proliferation-associated protein
• UniProt Gene Name: SIPA1
• UniProt Synonym Gene Names: SPA1; Sipa-1
• UniProt Entry Name: SIPA1_HUMAN

NCBI Description

The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date. [provided by RefSeq, Jul 2008]

Uniprot Description

SIPA1: GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting it to the putatively inactive GDP-bound state.

Protein type: GAPs; GAPs, Ras

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: Golgi apparatus; transport vesicle; protein complex; perinuclear region of cytoplasm; nucleolus; plasma membrane; cytosol; nucleus

Molecular Function: protein C-terminus binding; protein binding; GTPase activator activity

Biological Process: cell proliferation; cytoskeleton organization and biogenesis; cellular response to water deprivation; negative regulation of cell growth; negative regulation of cell adhesion; signal transduction; negative regulation of cell cycle; positive regulation of GTPase activity