Immunohistochemistry (IHC)
(Immunochemical staining of human SIPA1 in human kidney with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)
Rabbit anti-Human SIPA1 Polyclonal Antibody | anti-SIPA1 antibody
Anti-SIPA1 Antibody, Rabbit Polyclonal
IHC-P: 1:250-1:1000
ICC/IF: 1:300-1:10,000
Immunohistochemistry (IHC)
(Immunochemical staining of human SIPA1 in human kidney with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human SIPA1 in human kidney with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)
Immunofluorescence (IF)
(Immunofluorescence staining of SIPA1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human SIPA1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of SIPA1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human SIPA1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.)
NCBI and Uniprot Product Information
NCBI Description
The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date. [provided by RefSeq, Jul 2008]
Uniprot Description
SIPA1: GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting it to the putatively inactive GDP-bound state.
Protein type: GAPs, Ras; GAPs
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: Golgi apparatus; transport vesicle; protein complex; perinuclear region of cytoplasm; nucleolus; plasma membrane; cytosol; nucleus
Molecular Function: protein C-terminus binding; protein binding; GTPase activator activity
Biological Process: cell proliferation; cellular response to water deprivation; cytoskeleton organization and biogenesis; negative regulation of cell adhesion; negative regulation of cell growth; signal transduction; negative regulation of cell cycle; positive regulation of GTPase activity
Research Articles on SIPA1
Similar Products
Product Notes
The SIPA1 sipa1 (Catalog #AAA8100602) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SIPA1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SIPA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC-P: 1:250-1:1000 ICC/IF: 1:300-1:10,000. Researchers should empirically determine the suitability of the SIPA1 sipa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIPA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
