PSENEN sirna

PSENEN siRNA (Human)

Cat. Num. AAA8217145

• Gene Names: PSENEN; PEN2; PEN-2; MDS033; MSTP064
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PSENEN; PSENEN siRNA (Human); PEN2; Gamma-secretase subunit PEN-2; Presenilin enhancer protein 2; PSENEN sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PSENEN siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 101

• Applicable Applications for PSENEN sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PSENEN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PSENEN sirna

siRNA to inhibit PSENEN expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 528281416
• NCBI GeneID: 55851
• NCBI Accession #: NP_001268461.1
• NCBI GenBank Nucleotide #: NM_001281532.1
• UniProt Accession #: Q9NZ42; B2R5L9
• Molecular Weight: 12,029 Da
• NCBI Official Full Name: gamma-secretase subunit PEN-2
• NCBI Official Synonym Full Names: presenilin enhancer gamma secretase subunit
• NCBI Official Symbol: PSENEN
• NCBI Official Synonym Symbols: PEN2; PEN-2; MDS033; MSTP064
• NCBI Protein Information: gamma-secretase subunit PEN-2
• UniProt Protein Name: Gamma-secretase subunit PEN-2
• Protein Family: Probable gamma-secretase
• UniProt Gene Name: PSENEN
• UniProt Synonym Gene Names: PEN2
• UniProt Entry Name: PEN2_HUMAN

NCBI Description

Presenilins, which are components of the gamma-secretase protein complex, are required for intramembranous processing of some type I transmembrane proteins, such as the Notch proteins and the beta-amyloid precursor protein. Signaling by Notch receptors mediates a wide range of developmental cell fates. Processing of the beta-amyloid precursor protein generates neurotoxic amyloid beta peptides, the major component of senile plaques associated with Alzheimer's disease. This gene encodes a protein that is required for Notch pathway signaling, and for the activity and accumulation of gamma-secretase. Mutations resulting in haploinsufficiency for this gene cause familial acne inversa-2 (ACNINV2). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

PSENEN: Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta- amyloid precursor protein). Probably represents the last step of maturation of gamma-secretase, facilitating endoproteolysis of presenilin and conferring gamma-secretase activity. Component of the gamma-secretase complex, a complex composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PSENEN/PEN2. Such minimal complex is sufficient for secretase activity, although other components may exist. Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain. Belongs to the PEN-2 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.12

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; endoplasmic reticulum; integral to plasma membrane; plasma membrane

Molecular Function: protein binding

Biological Process: positive regulation of catalytic activity; axon guidance; Notch signaling pathway; amyloid precursor protein catabolic process; membrane protein intracellular domain proteolysis; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; membrane protein ectodomain proteolysis; ephrin receptor signaling pathway; Notch receptor processing; protein processing

Disease: Acne Inversa, Familial, 2