Rabbit anti-Human CTBP2 Polyclonal Antibody | anti-CTBP2 antibody

CTBP2, NT (CTBP2, C-terminal-binding protein 2) (Azide free) (HRP)

Cat. Num. AAA6289686

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CTBP2; Polyclonal Antibody; NT (CTBP2; C-terminal-binding protein 2) (Azide free) (HRP); C-terminal-binding protein 2; anti-CTBP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CTBP2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CTBP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CTBP2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CTBP2 (arrow) using rabbit polyclonal CTBP2 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CTBP2 gene.)

Immunohistochemistry (IHC)

(CTBP2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CTBP2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-CTBP2 antibody

This gene produces alternative transcripts encoding two distinct proteins. One protein is a transcriptional repressor, while the other isoform is a major component of specialized synapses known as synaptic ribbons. Both proteins contain a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases. A portion of the 3' untranslated region was used to map this gene to chromosome 21q21.3; however, it was noted that similar loci elsewhere in the genome are likely. Blast analysis shows that this gene is present on chromosome 10. [provided by RefSeq].

Product Categories/Family for anti-CTBP2 antibody

Antibodies; Binding Proteins

References

Bao, B.Y., et al. Clin. Chim. Acta 411 (17-18), 1232-1237 (2010): Liu, H., et al. Prostate (2010). In press: Kim, S.T., et al. Prostate (2010). In press: Gallagher, D.J., et al. Clin. Cancer Res. 16(10):2819-2832(2010). Kitamura, F., et al. Int. Arch. Allergy Immunol. 152 SUPPL 1, 18-21 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 145580575
• NCBI GeneID: 1488
• NCBI Accession #: NP_073713.2; NP_001320.1
• NCBI GenBank Nucleotide #: NM_022802.2
• UniProt Accession #: P56545; O43449; Q5SQP7; Q69YI3; Q86SV0; Q9H2T8; A8K2X5; D3DRF5
• Molecular Weight: 106,187 Da
• NCBI Official Full Name: C-terminal-binding protein 2 isoform 2
• NCBI Official Synonym Full Names: C-terminal binding protein 2
• NCBI Official Symbol: CTBP2
• NCBI Protein Information: C-terminal-binding protein 2
• UniProt Protein Name: C-terminal-binding protein 2
• Protein Family: C-terminal-binding protein
• UniProt Gene Name: CTBP2
• UniProt Synonym Gene Names: CtBP2
• UniProt Entry Name: CTBP2_HUMAN

NCBI Description

This gene produces alternative transcripts encoding two distinct proteins. One protein is a transcriptional repressor, while the other isoform is a major component of specialized synapses known as synaptic ribbons. Both proteins contain a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases. A portion of the 3' untranslated region was used to map this gene to chromosome 21q21.3; however, it was noted that similar loci elsewhere in the genome are likely. Blast analysis shows that this gene is present on chromosome 10. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]

Uniprot Description

CTBP2: Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation. Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 10q26.13

Cellular Component: transcriptional repressor complex; synapse; cell junction; nucleus

Molecular Function: protein binding; retinoic acid receptor binding; transcription coactivator activity; chromatin binding; NAD binding; transcription corepressor activity; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Biological Process: negative regulation of cell proliferation; transcription, DNA-dependent; white fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of retinoic acid receptor signaling pathway; viral genome replication; negative regulation of transcription, DNA-dependent