PDE3B sirna

PDE3B siRNA (Human)

Cat. Num. AAA8231342

• Gene Names: PDE3B; HcGIP1; cGIPDE1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PDE3B; PDE3B siRNA (Human); cGMP-inhibited 3'.5'-cyclic phosphodiesterase B; CGIPDE1; CGIP1; Cyclic GMP-inhibited phosphodiesterase B; CGI-PDE B; PDE3B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PDE3B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1112

• Applicable Applications for PDE3B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PDE3B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PDE3B sirna

siRNA to inhibit PDE3B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 40254434
• NCBI GeneID: 5140
• NCBI Accession #: NP_000913.2
• NCBI GenBank Nucleotide #: NM_000922.3
• UniProt Accession #: Q13370; O00639; Q14408; Q6SEI4; B7ZM37
• Molecular Weight: 118,809 Da
• NCBI Official Full Name: cGMP-inhibited 3',5'-cyclic phosphodiesterase B
• NCBI Official Synonym Full Names: phosphodiesterase 3B, cGMP-inhibited
• NCBI Official Symbol: PDE3B
• NCBI Official Synonym Symbols: HcGIP1; cGIPDE1
• NCBI Protein Information: cGMP-inhibited 3',5'-cyclic phosphodiesterase B
• UniProt Protein Name: cGMP-inhibited 3',5'-cyclic phosphodiesterase B
• Protein Family: cGMP-inhibited 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE3B
• UniProt Synonym Gene Names: CGIP1; CGI-PDE B
• UniProt Entry Name: PDE3B_HUMAN

Uniprot Description

PDE3B: a cGMP-inhibited phosphodiesterase 1 involved in fat metabolism. Inhibition of PDE3B can augment PDE4 inhibitor-induced apoptosis in some patients with chronic lymphocytic leukemia.

Protein type: Phosphodiesterase; Membrane protein, multi-pass; EC 3.1.4.17; Nucleotide Metabolism - purine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: Golgi apparatus; membrane; endoplasmic reticulum; integral to membrane; cytosol

Molecular Function: protein kinase B binding; protein binding; cGMP-inhibited cyclic-nucleotide phosphodiesterase activity; metal ion binding; 3',5'-cyclic-nucleotide phosphodiesterase activity

Biological Process: negative regulation of angiogenesis; negative regulation of lipid catabolic process; cellular response to insulin stimulus; cAMP catabolic process; insulin receptor signaling pathway; negative regulation of cell adhesion; angiogenesis; blood coagulation; glucose homeostasis; negative regulation of cell adhesion mediated by integrin; endocrine pancreas development; regulation of insulin secretion