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Western Blot (WB) (RAB3D Antibody (C-term) western blot analysis in K562,HL-60,MDA-MB231,MDA-MB453,CEM cell line lysates (35ug/lane).This demonstrates the RAB3D antibody detected the RAB3D protein (arrow).)

Rabbit anti-Human RAB3D Polyclonal Antibody | anti-RAB3D antibody

RAB3D, CT (RAB3D, GOV, RAB16, Ras-related protein Rab-3D) (Biotin)

Gene Names
RAB3D; GOV; D2-2; RAB16; RAD3D
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
RAB3D; Polyclonal Antibody; CT (RAB3D; GOV; RAB16; Ras-related protein Rab-3D) (Biotin); Ras-related protein Rab-3D; anti-RAB3D antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-RAB3D antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
ELISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
RAB3D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 184-212 amino acids from the C-terminal region of human RAB3D.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RAB3D Antibody (C-term) western blot analysis in K562,HL-60,MDA-MB231,MDA-MB453,CEM cell line lysates (35ug/lane).This demonstrates the RAB3D antibody detected the RAB3D protein (arrow).)

Western Blot (WB) (RAB3D Antibody (C-term) western blot analysis in K562,HL-60,MDA-MB231,MDA-MB453,CEM cell line lysates (35ug/lane).This demonstrates the RAB3D antibody detected the RAB3D protein (arrow).)

Immunohistochemistry (IHC)

(RAB3D Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RAB3D Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (RAB3D Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RAB3D Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(RAB3D Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (RAB3D Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-RAB3D antibody
RAB3D is a protein transport. Probably involved in regulated exocytosis (By similarity).
Product Categories/Family for anti-RAB3D antibody
References
Tian, X., et al. Mol. Cell. Biol. 30(5):1269-1284(2010). Knop, M., et al. EMBO J. 23(15):2982-2992(2004). Matsumoto, M., et al. Proc. Natl. Acad. Sci. U.S.A. 101(22):8313-8318(2004). Nguyen, D., et al. J. Cell. Physiol. 197(3):400-408(2003). Fukuda, M. J. Biol. Chem. 278(17):15373-15380(2003).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,267 Da
NCBI Official Full Name
ras-related protein Rab-3D
NCBI Official Synonym Full Names
RAB3D, member RAS oncogene family
NCBI Official Symbol
RAB3D
NCBI Official Synonym Symbols
GOV; D2-2; RAB16; RAD3D
NCBI Protein Information
ras-related protein Rab-3D; Rab3D upregulated with myeloid differentiation; glioblastoma overexpressed
UniProt Protein Name
Ras-related protein Rab-3D
Protein Family
UniProt Gene Name
RAB3D
UniProt Synonym Gene Names
GOV; RAB16
UniProt Entry Name
RAB3D_HUMAN

Uniprot Description

RAB3D: Protein transport. Probably involved in regulated exocytosis. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: synaptic vesicle; transport vesicle; mitochondrion; secretory granule membrane; plasma membrane; endosome

Molecular Function: GTPase activity; GTPase binding; GDP binding; GTP binding; myosin V binding

Biological Process: regulation of exocytosis; intracellular protein transport; synaptic vesicle exocytosis; protein secretion; peptidyl-cysteine methylation; Rab protein signal transduction; vesicle docking during exocytosis

Research Articles on RAB3D

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Product Notes

The RAB3D rab3d (Catalog #AAA6339780) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAB3D, CT (RAB3D, GOV, RAB16, Ras-related protein Rab-3D) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAB3D can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 ELISA: 1:1,000 IHC: 1:10-50 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RAB3D rab3d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB3D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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