Western Blot (WB)
(Western blot analysis of DIRAS3 expression in 293 lysate, The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human DIRAS3 Polyclonal Antibody | anti-DIRAS3 antibody
DIRAS3 Antibody
Gene Names
DIRAS3; ARHI; NOEY2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
DIRAS3; Polyclonal Antibody; DIRAS3 Antibody; DIRA3; DIRA3_HUMAN; DIRAS family GTP binding RAS like 3; DIRAS family GTPase 3; DIRAS family; GTP-binding RAS-like protein 3; Distinct subgroup of the Ras family member 3; GTP binding protein Di Ras3; GTP-binding protein Di-Ras3; NOEY2; Ras homolog gene family member I; Rho related GTP binding protein RhoI; Rho-related GTP-binding protein RhoI; RHOI; anti-DIRAS3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
DIRAS3 antibody detects endogenous levels of total DIRAS3
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
229
Applicable Applications for anti-DIRAS3 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide
Subcellular Location
Cell Membrane
Tissue Specificity
Expressed in normal ovarian and breast epithelial cells but not in ovarian and breast cancers.
Conjugation
Unconjugated
Western Blot (WB)
(Western blot analysis of DIRAS3 expression in 293 lysate, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of DIRAS3 expression in 293 lysate, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of DIRAS3 Antibody expression in 293 cells lysates.)
Western Blot (WB)
(Western blot analysis of DIRAS3 Antibody expression in 293 cells lysates.)
Immunohistochemistry (IHC)
(MBS9603738 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603738 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-DIRAS3 antibody
Description: Expressed in normal ovarian and breast epithelial cells but not in ovarian and breast cancers.
Similarity: Belongs to the small GTPase superfamily. Di-Ras family.
Similarity: Belongs to the small GTPase superfamily. Di-Ras family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 26 kDa
Predicted: 26 kDa
Predicted: 26 kDa
NCBI Official Full Name
GTP-binding protein Di-Ras3
NCBI Official Synonym Full Names
DIRAS family GTPase 3
NCBI Official Symbol
DIRAS3
NCBI Official Synonym Symbols
ARHI; NOEY2
NCBI Protein Information
GTP-binding protein Di-Ras3
UniProt Protein Name
GTP-binding protein Di-Ras3
Protein Family
UniProt Gene Name
DIRAS3
UniProt Synonym Gene Names
ARHI; NOEY2; RHOI
NCBI Description
This gene encodes a member of the ras superfamily. This gene is imprinted gene with monoallelic expression of the paternal allele which is associated with growth suppression. The encoded protein acts as a tumor suppressor whose function is abrogated in many ovarian and breast cancers. This protein may also play a role autophagy in certain cancer cells by regulating the autophagosome initiation complex. [provided by RefSeq, Nov 2015]
Research Articles on DIRAS3
Similar Products
Product Notes
The DIRAS3 diras3 (Catalog #AAA9603738) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DIRAS3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DIRAS3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the DIRAS3 diras3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DIRAS3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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