Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PDE3B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 40s.)
Rabbit anti-Human, Mouse PDE3B Polyclonal Antibody | anti-PDE3B antibody
PDE3B Polyclonal Antibody
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PDE3B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 40s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PDE3B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 40s.)
NCBI and Uniprot Product Information
Uniprot Description
PDE3B: a cGMP-inhibited phosphodiesterase 1 involved in fat metabolism. Inhibition of PDE3B can augment PDE4 inhibitor-induced apoptosis in some patients with chronic lymphocytic leukemia.
Protein type: Membrane protein, multi-pass; EC 3.1.4.17; Membrane protein, integral; Nucleotide Metabolism - purine; Phosphodiesterase
Chromosomal Location of Human Ortholog: 11p15.1
Cellular Component: Golgi apparatus; membrane; endoplasmic reticulum; integral to membrane; cytosol
Molecular Function: protein kinase B binding; protein binding; cGMP-inhibited cyclic-nucleotide phosphodiesterase activity; metal ion binding; 3',5'-cyclic-nucleotide phosphodiesterase activity
Biological Process: negative regulation of angiogenesis; cellular response to insulin stimulus; negative regulation of lipid catabolic process; cAMP catabolic process; insulin receptor signaling pathway; negative regulation of cell adhesion; angiogenesis; blood coagulation; glucose homeostasis; endocrine pancreas development; regulation of insulin secretion; negative regulation of cell adhesion mediated by integrin
Research Articles on PDE3B
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Product Notes
The PDE3B pde3b (Catalog #AAA9128645) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDE3B Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PDE3B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB 1:500 - 1:2000. Researchers should empirically determine the suitability of the PDE3B pde3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDE3B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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