OSTM1 sirna

OSTM1 siRNA (Human)

Cat. Num. AAA8232851

• Gene Names: OSTM1; GL; GIPN; OPTB5; HSPC019
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: OSTM1; OSTM1 siRNA (Human); GL; Osteopetrosis-associated transmembrane protein 1; Chloride channel 7 beta subunit; OSTM1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: OSTM1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 334

• Applicable Applications for OSTM1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human OSTM1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for OSTM1 sirna

siRNA to inhibit OSTM1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 30025026
• NCBI GeneID: 28962
• NCBI Accession #: NP_054747.2
• NCBI GenBank Nucleotide #: NM_014028.3
• UniProt Accession #: Q86WC4; Q5R391; Q6PCA7; Q7RTW6; Q8NC29; Q8TC82; Q9Y2S9; E1P5E3
• Molecular Weight: 37,257 Da
• NCBI Official Full Name: osteopetrosis-associated transmembrane protein 1
• NCBI Official Synonym Full Names: osteopetrosis associated transmembrane protein 1
• NCBI Official Symbol: OSTM1
• NCBI Official Synonym Symbols: GL; GIPN; OPTB5; HSPC019
• NCBI Protein Information: osteopetrosis-associated transmembrane protein 1
• UniProt Protein Name: Osteopetrosis-associated transmembrane protein 1
• Protein Family: Osteopetrosis-associated transmembrane protein
• UniProt Gene Name: OSTM1
• UniProt Synonym Gene Names: GL
• UniProt Entry Name: OSTM1_HUMAN

NCBI Description

This gene encodes a protein that may be involved in the degradation of G proteins via the ubiquitin-dependent proteasome pathway. The encoded protein binds to members of subfamily A of the regulator of the G-protein signaling (RGS) family through an N-terminal leucine-rich region. This protein also has a central RING finger-like domain and E3 ubiquitin ligase activity. This protein is highly conserved from flies to humans. Defects in this gene may cause the autosomal recessive, infantile malignant form of osteopetrosis. [provided by RefSeq, Jul 2008]

Uniprot Description

OSTM1: Required for osteoclast and melanocyte maturation and function. Defects in OSTM1 are the cause of osteopetrosis autosomal recessive type 5 (OPTB5); also called infantile malignant osteopetrosis 3. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. OPTB5 patients manifest primary central nervous system involvement in addition to the classical stigmata of severe bone sclerosis, growth failure, anemia, thrombocytopenia and visual impairment with optic atrophy.

Protein type: Membrane protein, integral; Ubiquitin conjugating system; Ligase; EC 6.3.2.-; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: intracellular membrane-bound organelle; lysosomal membrane; cytoplasm; integral to membrane; nucleus; cytosol

Biological Process: osteoclast differentiation; transmembrane transport

Disease: Osteopetrosis, Autosomal Recessive 5