OSTM1 recombinant protein

OSTM1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120576

• Gene Names: OSTM1; GL; GIPN; OPTB5; HSPC019
• Purity: >97% as determined by SDS-PAGE
• Synonyms: OSTM1; OSTM1 Protein; Human; Recombinant (His Tag); Human OSTM1 Protein (His Tag); GIPN Protein; GL Protein; HSPC019 Protein; OPTB5 Protein; osteopetrosis associated transmembrane protein 1; OSTM1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Pro284

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 32
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain of human OSTM1 (NP_054747.2)(Met 1-Pro 284) was expressed, fused with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for OSTM1 recombinant protein

Background: Osteopetrosis-associated transmembrane protein 1 (OSTM1) is a Single-pass type I membrane protein. It is expressed in many hematopoietic cells of the myeloid and lymphoid B- and T-lineages. The analysis of OSTM1 association with CLCN7 demonstrated that OSTM1 requires CLCN7 to localize to lysosomes, whereas the formation of a CLCN7-OSTM1 complex is required to stabilize CLCN7. OSTM1 plays a major role in myelopoiesis and lymphopoiesis and provided evidence of a crosstalk mechanism between hematopoietic cells for osteoclast activation. Thus, OSTM1 has an important role in osteoclast function and activation. The loss of function of OSTM1 results in deregulation of multiple hematopoietic lineages in addition to osteoclast lineage, OSTM1-defect patients display the most severe recessive osteopetrosis phenotype and die at early ages. Furthermore, it is suggested that OSTM1 has a primary role in neural development not related to lysosomal dysfunction. The canonical Wnt/beta-catenin signaling pathway may be a molecular basis for OSTM1 mutations and severe autosomal recessive osteopetrosis (ARO).

References

1. Chalhoub, N. et al., 2003, Nat Med. 9 (4): 399-406. 2. Quarello, P. et al., 2004, J Bone Miner Res. 19 (7): 1194-1199. 3. Lange, PF. et al., 2006, Nature. 440 (7081): 220-223 4. Maranda, B. et al., 2008, J Bone Miner Res. 23 (2): 296-300. 5. Feigin, ME.et al., 2008, Cell Signal. 20 (5): 949-957. 6. Pata, M.et al., 2008, J Biol Chem. 283 (45): 30522-30530.

NCBI and Uniprot Product Information

• NCBI GI #: 30025026
• NCBI GeneID: 28962
• NCBI Accession #: NP_054747.2
• NCBI GenBank Nucleotide #: NM_014028.3
• UniProt Accession #: Q86WC4; Q5R391; Q6PCA7; Q7RTW6; Q8NC29; Q8TC82; Q9Y2S9; E1P5E3
• Molecular Weight: 37,257 Da
• NCBI Official Full Name: osteopetrosis-associated transmembrane protein 1
• NCBI Official Synonym Full Names: osteopetrosis associated transmembrane protein 1
• NCBI Official Symbol: OSTM1
• NCBI Official Synonym Symbols: GL; GIPN; OPTB5; HSPC019
• NCBI Protein Information: osteopetrosis-associated transmembrane protein 1
• UniProt Protein Name: Osteopetrosis-associated transmembrane protein 1
• Protein Family: Osteopetrosis-associated transmembrane protein
• UniProt Gene Name: OSTM1
• UniProt Synonym Gene Names: GL
• UniProt Entry Name: OSTM1_HUMAN

NCBI Description

This gene encodes a protein that may be involved in the degradation of G proteins via the ubiquitin-dependent proteasome pathway. The encoded protein binds to members of subfamily A of the regulator of the G-protein signaling (RGS) family through an N-terminal leucine-rich region. This protein also has a central RING finger-like domain and E3 ubiquitin ligase activity. This protein is highly conserved from flies to humans. Defects in this gene may cause the autosomal recessive, infantile malignant form of osteopetrosis. [provided by RefSeq, Jul 2008]