APLP1 recombinant protein

APLP1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121514

• Gene Names: APLP1; APLP
• Purity: >95% as determined by SDS-PAGE
• Synonyms: APLP1; APLP1 Protein; Human; Recombinant (His Tag); Human APLP1/Amyloid-like protein 1 Protein (His Tag); APLP Protein; amyloid beta (A4) precursor-like protein 1; APLP1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Glu580

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser 39
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human APLP1 (P51693-1)(Met1-Glu580) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for APLP1 recombinant protein

Background: APLP1, also known as amyloid-like protein 1, is a member of the highly conserved amyloid precursor protein gene family. APLP1 is a membrane-associated glycoprotein that is cleaved by secretases in a manner similar to amyloid beta A4 precursor protein cleavage. APLP1, together with APLP2, are important modulators of glucose. APLP1 may also play a role in synaptic maturation during cortical development. Alternatively spliced transcript variants encoding different isoforms have been described. APLP1 also is a mammalian homologue of amyloid precursor protein (APP). APP is a type I membrane protein that is genetically linked to Alzheimer's disease.

References

Wasco W. et al., 1993, Genomics. 15 (1): 237-9. Needham BE. et al., 2008, J Pathol. 215 (2): 155-63. Bayer TA. et al., 2000, Mol Psychiatry. 4 (6): 524-8. Lee S. et al., 2011, Biochemistry. 50 (24): 5453-64.

NCBI and Uniprot Product Information

• NCBI GI #: 28558769
• NCBI GeneID: 333
• UniProt Accession #: P51693; O00113; Q96A92
• Molecular Weight: 650
• NCBI Official Full Name: Amyloid-like protein 1
• NCBI Official Synonym Full Names: amyloid beta (A4) precursor-like protein 1
• NCBI Official Symbol: APLP1
• NCBI Official Synonym Symbols: APLP
• NCBI Protein Information: amyloid-like protein 1; APLP-1; amyloid precursor-like protein 1
• UniProt Protein Name: Amyloid-like protein 1
• Protein Family: Amyloid-like protein
• UniProt Gene Name: APLP1
• UniProt Synonym Gene Names: APLP; APLP-1
• UniProt Entry Name: APLP1_HUMAN

NCBI Description

This gene encodes a member of the highly conserved amyloid precursor protein gene family. The encoded protein is a membrane-associated glycoprotein that is cleaved by secretases in a manner similar to amyloid beta A4 precursor protein cleavage. This cleavage liberates an intracellular cytoplasmic fragment that may act as a transcriptional activator. The encoded protein may also play a role in synaptic maturation during cortical development. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

APLP1: May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding. Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I. Belongs to the APP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; integral to membrane; plasma membrane; basement membrane

Molecular Function: heparin binding; identical protein binding; protein binding; alpha-2C adrenergic receptor binding; transition metal ion binding; alpha-2B adrenergic receptor binding; alpha-2A adrenergic receptor binding

Biological Process: regulation of translation; nervous system development; negative regulation of cAMP biosynthetic process; organ morphogenesis; extracellular matrix organization and biogenesis; apoptosis; forebrain development; mRNA polyadenylation; endocytosis; G-protein signaling, adenylate cyclase inhibiting pathway; cell adhesion