JAG2 sirna

JAG2 siRNA (Human)

Cat. Num. AAA8236406

• Gene Names: JAG2; HJ2; SER2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: JAG2; JAG2 siRNA (Human); Protein jagged-2; Jagged2; hJ2; JAG2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: JAG2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,200

• Applicable Applications for JAG2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human JAG2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for JAG2 sirna

siRNA to inhibit JAG2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 21704277
• NCBI GeneID: 3714
• NCBI Accession #: NP_002217.3
• NCBI GenBank Nucleotide #: NM_002226.4
• UniProt Accession #: Q9Y219; Q9UE17; Q9UE99; Q9UNK8; Q9Y6P9; Q9Y6Q0
• Molecular Weight: 129,235 Da
• NCBI Official Full Name: protein jagged-2 isoform a
• NCBI Official Synonym Full Names: jagged 2
• NCBI Official Symbol: JAG2
• NCBI Official Synonym Symbols: HJ2; SER2
• NCBI Protein Information: protein jagged-2
• UniProt Protein Name: Protein jagged-2
• Protein Family: Protein jagged
• UniProt Gene Name: JAG2
• UniProt Synonym Gene Names: Jagged2; hJ2
• UniProt Entry Name: JAG2_HUMAN

NCBI Description

The Notch signaling pathway is an intercellular signaling mechanism that is essential for proper embryonic development. Members of the Notch gene family encode transmembrane receptors that are critical for various cell fate decisions. The protein encoded by this gene is one of several ligands that activate Notch and related receptors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

JAG2: Putative Notch ligand involved in the mediation of Notch signaling. Involved in limb development. Expressed in heart, placenta and skeletal muscle and to a lesser extend in pancreas. Very low expression in brain, lung, liver and kidney. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 14q32

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; growth factor activity; calcium ion binding; Notch binding

Biological Process: regulation of cell adhesion; thymic T cell selection; Notch signaling pathway; in utero embryonic development; Notch receptor processing; cell cycle; regulation of cell migration; odontogenesis of dentine-containing teeth; regulation of cell proliferation; respiratory system process; morphogenesis of embryonic epithelium; spermatogenesis; auditory receptor cell fate commitment; cell differentiation; skeletal development; T cell differentiation; gamma-delta T cell differentiation