Jagged 2 Protein (JAG2) Recombinant Protein | JAG2 recombinant protein

Recombinant Jagged 2 Protein (JAG2)

Cat. Num. AAA2011152

• Gene Names: JAG2; HJ2; SER2
• Applications: SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: Jagged 2 Protein (JAG2); Recombinant Jagged 2 Protein (JAG2); JAG2 recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Concentration: Original Concentration: 200 ug/mL (varies by lot)

• Applicable Applications for JAG2 recombinant protein: Positive Control; Immunogen; SDS-PAGE; WB.; ; (May be suitable for use in other assays to be determined by the end user.)
• Organism Species: Homo sapiens (Human)
• Source: Prokaryotic expression
• Residues: Phe714~His981
• Tags: N-terminal His Tag
• Subcellular Location: Membrane
• Traits: Freeze-dried powder
• Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Predicted isoelectric point: 6.6
• Predicted Molecular Mass: 32.4kDa
• Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles.; Store at 2-8ºC for one month.; Aliquot and store at -80ºC for 12 months.; ; Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 21704277
• NCBI GeneID: 3714
• NCBI Accession #: NP_002217.3
• NCBI GenBank Nucleotide #: NM_002226.4
• UniProt Accession #: Q9Y219; Q9UE17; Q9UE99; Q9UNK8; Q9Y6P9; Q9Y6Q0
• NCBI Official Full Name: protein jagged-2 isoform a
• NCBI Official Synonym Full Names: jagged 2
• NCBI Official Symbol: JAG2
• NCBI Official Synonym Symbols: HJ2; SER2
• NCBI Protein Information: protein jagged-2
• UniProt Protein Name: Protein jagged-2
• Protein Family: Protein jagged
• UniProt Gene Name: JAG2
• UniProt Synonym Gene Names: Jagged2; hJ2
• UniProt Entry Name: JAG2_HUMAN

NCBI Description

The Notch signaling pathway is an intercellular signaling mechanism that is essential for proper embryonic development. Members of the Notch gene family encode transmembrane receptors that are critical for various cell fate decisions. The protein encoded by this gene is one of several ligands that activate Notch and related receptors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

JAG2: Putative Notch ligand involved in the mediation of Notch signaling. Involved in limb development. Expressed in heart, placenta and skeletal muscle and to a lesser extend in pancreas. Very low expression in brain, lung, liver and kidney. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 14q32

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; growth factor activity; calcium ion binding; Notch binding

Biological Process: regulation of cell adhesion; thymic T cell selection; Notch signaling pathway; in utero embryonic development; Notch receptor processing; cell cycle; regulation of cell migration; odontogenesis of dentine-containing teeth; regulation of cell proliferation; respiratory system process; morphogenesis of embryonic epithelium; spermatogenesis; cell differentiation; auditory receptor cell fate commitment; skeletal development; gamma-delta T cell differentiation; T cell differentiation