HMGA2 sirna

HMGA2 siRNA (Human)

Cat. Num. AAA8238557

• Gene Names: HMGA2; BABL; LIPO; HMGIC; HMGI-C; STQTL9
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HMGA2; HMGA2 siRNA (Human); HMGIC; High mobility group protein HMGI-C; High mobility group AT-hook protein 2; HMGA2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: HMGA2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 109

• Applicable Applications for HMGA2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HMGA2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HMGA2 sirna

siRNA to inhibit HMGA2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4504431
• NCBI GeneID: 8091
• NCBI Accession #: NP_003474.1
• NCBI GenBank Nucleotide #: NM_003483.4
• UniProt Accession #: P52926; Q1M182; Q1M185; Q1M186; Q1M187; Q1M188; E7EP85; E7EWA2
• Molecular Weight: 9,742 Da
• NCBI Official Full Name: high mobility group protein HMGI-C isoform a
• NCBI Official Synonym Full Names: high mobility group AT-hook 2
• NCBI Official Symbol: HMGA2
• NCBI Official Synonym Symbols: BABL; LIPO; HMGIC; HMGI-C; STQTL9
• NCBI Protein Information: high mobility group protein HMGI-C
• UniProt Protein Name: High mobility group protein HMGI-C
• Protein Family: High mobility group protein
• UniProt Gene Name: HMGA2
• UniProt Synonym Gene Names: HMGIC
• UniProt Entry Name: HMGA2_HUMAN

NCBI Description

This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

HMGA2: Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes. Interacts with E4F1. Interacts with NEK2. Belongs to the HMGA family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Oncoprotein

Chromosomal Location of Human Ortholog: 12q15

Cellular Component: nucleoplasm; nuclear chromosome; nucleus

Molecular Function: protein binding; DNA-(apurinic or apyrimidinic site) lyase activity; nucleosomal DNA binding; DNA binding; AT DNA binding; DNA-dependent protein kinase activity; 5'-deoxyribose-5-phosphate lyase activity; SMAD binding; transcription factor binding; DNA bending activity

Biological Process: fat cell differentiation; establishment and/or maintenance of chromatin architecture; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; multicellular organismal development; mesodermal cell differentiation; negative regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; base-excision repair; regulation of growth; chondrocyte differentiation; negative regulation of retroviral genome replication; mitosis; transcription, DNA-dependent; response to virus; heterochromatin formation; DNA damage response, detection of DNA damage; stem cell differentiation; DNA catabolic process, endonucleolytic; negative regulation of DNA binding; chromosome condensation; mesenchymal cell differentiation; cell division; mitotic cell cycle G2/M transition DNA damage checkpoint; chromosome breakage; epithelial to mesenchymal transition; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis

Disease: Leiomyoma, Uterine