SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
ACKA recombinant protein
ACKA Protein
Gene Names
ackA; ECK2290; JW2293
Applications
Western Blot
Synonyms
ACKA; ACKA Protein; ECK2290; JW2293; ACKA recombinant protein
Host
E Coli
Form/Format
Recombinant protein stored in 50mM sodium phosphate, pH7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 2mM DTT, 25% glycerol.
Applicable Applications for ACKA recombinant protein
Western Blot (WB)
Tag
HIS
Species
Bacteria
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for ACKA recombinant protein
Full-length recombinant bacterial ACKA was expressed in E Coli cells using an N-terminal His tag. The protein accession number is NP_416799.
ACKA belongs to the ASKHA superfamily of phosphor-transferases, and is the prototypic carboxylate kinase. Widely distributed in the bacteria and archaea domains. Catalyzes the phosphorylation of acetate.
ACKA belongs to the ASKHA superfamily of phosphor-transferases, and is the prototypic carboxylate kinase. Widely distributed in the bacteria and archaea domains. Catalyzes the phosphorylation of acetate.
Product Categories/Family for ACKA recombinant protein
References
1. Buss KA, et al: (2001) Urkinase: structure of acetate kinase, a member of the ASKHA superfamily of phosphotransferases. J Bacteriol. 183(2):680-686.
2. http://www.uniprot.org/uniprot/F3MTX6
2. http://www.uniprot.org/uniprot/F3MTX6
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45.7kDa (423aa), confirmed by MALDI-TOF
NCBI Official Full Name
acetate kinase A and propionate kinase 2
NCBI Official Symbol
ackA
NCBI Official Synonym Symbols
ECK2290; JW2293
NCBI Protein Information
acetate kinase A and propionate kinase 2
UniProt Protein Name
Acetate kinase
Protein Family
UniProt Gene Name
ackA
NCBI Description
N-terminus is blocked (Fox, 86). [More information is available at EcoGene: EG10027]. Regulation has been described . [More information is available at EcoCyc: EG10027].
Uniprot Description
Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction. During anaerobic growth of the organism, this enzyme is also involved in the synthesis of most of the ATP formed catabolically.
Research Articles on ACKA
Similar Products
Product Notes
The ACKA acka (Catalog #AAA516526) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ACKA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the ACKA acka for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACKA, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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