Human High mobility group protein HMGI-C ELISA Kit | HMGA2 elisa kit

Human High mobility group protein HMGI-C ELISA Kit

Cat. Num. AAA763117

• Gene Names: HMGA2; BABL; LIPO; HMGIC; HMGI-C; STQTL9
• Reactivity: Human
• Synonyms: High mobility group protein HMGI-C; Human High mobility group protein HMGI-C ELISA Kit; HMGA2 (High-mobility group protein HMGI-C)/BABL/HMGIC/LIPO/HMGI-C/STQTL9/high mobility group AT-hook 2/High mobility group AT-hook protein 2/high mobility group protein/high-mobility group (nonhistone chromosomal) protein isoform I-C; HMGA2 elisa kit

• Reactivity: Human
• Sequence Length: 118

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for HMGA2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human HMGA2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human HMGA2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human HMGA2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human HMGA2can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 665506020
• NCBI GeneID: 8091
• NCBI Accession #: NP_001287847.1
• NCBI GenBank Nucleotide #: NM_001300918.1
• Molecular Weight: 9,742 Da
• NCBI Official Full Name: high mobility group protein HMGI-C isoform c
• NCBI Official Synonym Full Names: high mobility group AT-hook 2
• NCBI Official Symbol: HMGA2
• NCBI Official Synonym Symbols: BABL; LIPO; HMGIC; HMGI-C; STQTL9
• NCBI Protein Information: high mobility group protein HMGI-C
• UniProt Protein Name: High mobility group protein HMGI-C
• Protein Family: High mobility group protein
• UniProt Gene Name: HMGA2
• UniProt Synonym Gene Names: HMGIC
• UniProt Entry Name: HMGA2_HUMAN

NCBI Description

This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

HMGA2: Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes. Interacts with E4F1. Interacts with NEK2. Belongs to the HMGA family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Oncoprotein

Chromosomal Location of Human Ortholog: 12q15

Cellular Component: nuclear chromosome; nucleoplasm; nucleus

Molecular Function: 5'-deoxyribose-5-phosphate lyase activity; AT DNA binding; DNA bending activity; DNA binding; DNA-(apurinic or apyrimidinic site) lyase activity; DNA-dependent protein kinase activity; nucleosomal DNA binding; protein binding; SMAD binding; transcription factor binding

Biological Process: base-excision repair; cell division; chondrocyte differentiation; chromosome breakage; chromosome condensation; DNA damage response, detection of DNA damage; epithelial to mesenchymal transition; establishment and/or maintenance of chromatin architecture; fat cell differentiation; heterochromatin formation; mesenchymal cell differentiation; mesodermal cell differentiation; mitosis; mitotic cell cycle G2/M transition DNA damage checkpoint; multicellular organismal development; negative regulation of apoptosis; negative regulation of DNA binding; negative regulation of retroviral genome replication; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of growth; regulation of transcription, DNA-dependent; response to virus; stem cell differentiation; transcription from RNA polymerase II promoter

Disease: Leiomyoma, Uterine