COL9A3 sirna

COL9A3 siRNA (Human)

Cat. Num. AAA8213100

• Gene Names: COL9A3; IDD; MED; EDM3; DJ885L7.4.1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: COL9A3; COL9A3 siRNA (Human); Collagen alpha-3(IX) chain; COL9A3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: COL9A3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 684

• Applicable Applications for COL9A3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human COL9A3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for COL9A3 sirna

siRNA to inhibit COL9A3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 119508426
• NCBI GeneID: 1299
• NCBI Accession #: NP_001844.3
• NCBI GenBank Nucleotide #: NM_001853.3
• UniProt Accession #: Q14050; Q13681; Q9H4G9; Q9UPE2
• Molecular Weight: 63,616 Da
• NCBI Official Full Name: collagen alpha-3(IX) chain
• NCBI Official Synonym Full Names: collagen, type IX, alpha 3
• NCBI Official Symbol: COL9A3
• NCBI Official Synonym Symbols: IDD; MED; EDM3; DJ885L7.4.1
• NCBI Protein Information: collagen alpha-3(IX) chain
• UniProt Protein Name: Collagen alpha-3(IX) chain
• Protein Family: Collagen
• UniProt Gene Name: COL9A3
• UniProt Entry Name: CO9A3_HUMAN

NCBI Description

This gene encodes one of the three alpha chains of type IX collagen, the major collagen component of hyaline cartilage. Type IX collagen, a heterotrimeric molecule, is usually found in tissues containing type II collagen, a fibrillar collagen. Mutations in this gene are associated with multiple epiphyseal dysplasia type 3. [provided by RefSeq, Jan 2010]

Uniprot Description

COL9A3: Structural component of hyaline cartilage and vitreous of the eye. Defects in COL9A3 are the cause of multiple epiphyseal dysplasia type 3 (EDM3); also known as multiple epiphyseal dysplasia with myopathy. EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types. Defects in COL9A3 are a cause of susceptibility to intervertebral disk disease (IDD). A common musculo- skeletal disorder caused by degeneration of intervertebral disks of the lumbar spine. It results in low-back pain and unilateral leg pain. Susceptibility to intervertebral disk disease, is conferred by variant p.Arg103Trp (PubMed:11308397). Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: proteinaceous extracellular matrix; endoplasmic reticulum lumen; extracellular region

Molecular Function: extracellular matrix structural constituent conferring tensile strength

Biological Process: axon guidance; collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; male gonad development; female gonad development

Disease: Epiphyseal Dysplasia, Multiple, 3; Intervertebral Disc Disease