Human Collagen alpha-3(IX) chain ELISA Kit | COL9A3 elisa kit

Human Collagen alpha-3(IX) chain ELISA Kit

Cat. Num. AAA765153

• Gene Names: COL9A3; IDD; MED; EDM3; DJ885L7.4.1
• Reactivity: Human
• Synonyms: Collagen alpha-3(IX) chain; Human Collagen alpha-3(IX) chain ELISA Kit; COL9A3/Collagen alpha-3 (IX) chain/COL9alpha3/EDM3/IDD/MED; COL9A3 elisa kit

• Reactivity: Human
• Sequence Length: 684

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.781-50ng/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for COL9A3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human COL9A3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human COL9A3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human COL9A3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration o fHuman COL9A3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 119508426
• NCBI GeneID: 1299
• NCBI Accession #: NP_001844.3
• NCBI GenBank Nucleotide #: NM_001853.3
• Molecular Weight: 63,616 Da
• NCBI Official Full Name: collagen alpha-3(IX) chain
• NCBI Official Synonym Full Names: collagen type IX alpha 3
• NCBI Official Symbol: COL9A3
• NCBI Official Synonym Symbols: IDD; MED; EDM3; DJ885L7.4.1
• NCBI Protein Information: collagen alpha-3(IX) chain
• UniProt Protein Name: Collagen alpha-3(IX) chain
• Protein Family: Collagen
• UniProt Gene Name: COL9A3
• UniProt Entry Name: CO9A3_HUMAN

NCBI Description

This gene encodes one of the three alpha chains of type IX collagen, the major collagen component of hyaline cartilage. Type IX collagen, a heterotrimeric molecule, is usually found in tissues containing type II collagen, a fibrillar collagen. Mutations in this gene are associated with multiple epiphyseal dysplasia type 3. [provided by RefSeq, Jan 2010]

Uniprot Description

COL9A3: Structural component of hyaline cartilage and vitreous of the eye. Defects in COL9A3 are the cause of multiple epiphyseal dysplasia type 3 (EDM3); also known as multiple epiphyseal dysplasia with myopathy. EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types. Defects in COL9A3 are a cause of susceptibility to intervertebral disk disease (IDD). A common musculo- skeletal disorder caused by degeneration of intervertebral disks of the lumbar spine. It results in low-back pain and unilateral leg pain. Susceptibility to intervertebral disk disease, is conferred by variant p.Arg103Trp (PubMed:11308397). Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: endoplasmic reticulum lumen; extracellular region; proteinaceous extracellular matrix

Molecular Function: extracellular matrix structural constituent conferring tensile strength

Biological Process: axon guidance; collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; female gonad development; male gonad development

Disease: Epiphyseal Dysplasia, Multiple, 3; Intervertebral Disc Disease