Rabbit anti-Human, Mouse DPH2 Polyclonal Antibody | anti-DPH2 antibody

DPH2 Rabbit Polyclonal

Cat. Num. AAA768748

• Gene Names: DPH2; DPH2L2
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: DPH2; Polyclonal Antibody; DPH2 Rabbit Polyclonal; anti-DPH2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 489

• Applicable Applications for anti-DPH2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)
• Immunogen: DPH2 homolog (S. cerevisiae)
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon cancer using MBS768748 (DPH2 antibody) at dilution of 1:50)

Testing Data

()

Western Blot (WB)

(human brain tissue were subjected to SDS PAGE followed by western blot with MBS768748 (DPH2 antibody) at dilution of 1:400)

NCBI and Uniprot Product Information

• NCBI GI #: 3170458
• NCBI GeneID: 1802
• Molecular Weight: 38,510 Da
• NCBI Official Full Name: diphthamide biosynthesis protein-2
• NCBI Official Synonym Full Names: DPH2 homolog
• NCBI Official Symbol: DPH2
• NCBI Official Synonym Symbols: DPH2L2
• NCBI Protein Information: diphthamide biosynthesis protein 2
• UniProt Protein Name: Diphthamide biosynthesis protein 2
• Protein Family: 2-(3-amino-3-carboxypropyl)histidine synthase
• UniProt Gene Name: DPH2
• UniProt Synonym Gene Names: DPH2L2; HsDph2; DPH2-like 2
• UniProt Entry Name: DPH2_HUMAN

NCBI Description

This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]

Uniprot Description

DPH2: Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2. Belongs to the DPH1/DPH2 family. DPH2 subfamily.

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: cytosol

Molecular Function: protein binding

Biological Process: peptidyl-diphthamide biosynthetic process from peptidyl-histidine