ATP8B2 sirna

ATP8B2 siRNA (Human)

Cat. Num. AAA8227587

• Gene Names: ATP8B2; ATPID
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ATP8B2; ATP8B2 siRNA (Human); ATPID; KIAA1137; Probable phospholipid-transporting ATPase ID; ATPase class I type 8B member 2; ATP8B2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ATP8B2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 387

• Applicable Applications for ATP8B2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ATP8B2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ATP8B2 sirna

siRNA to inhibit ATP8B2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 55743077
• NCBI GeneID: 57198
• NCBI Accession #: NP_001005855.1
• NCBI GenBank Nucleotide #: NM_001005855.1
• UniProt Accession #: P98198; Q6NT69; Q7Z486; Q96I43; Q96NQ7; B4E3P4
• Molecular Weight: 44,213 Da
• NCBI Official Full Name: phospholipid-transporting ATPase ID isoform b
• NCBI Official Synonym Full Names: ATPase, aminophospholipid transporter, class I, type 8B, member 2
• NCBI Official Symbol: ATP8B2
• NCBI Official Synonym Symbols: ATPID
• NCBI Protein Information: phospholipid-transporting ATPase ID
• UniProt Protein Name: Phospholipid-transporting ATPase ID
• Protein Family: Phospholipid-transporting ATPase
• UniProt Gene Name: ATP8B2
• UniProt Synonym Gene Names: ATPID; KIAA1137
• UniProt Entry Name: AT8B2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of aminophospholipid-transporting ATPases. The aminophospholipid translocases transport phosphatidylserine and phosphatidylethanolamine from one side of a bilayer to another. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP8B2: belongs to the family of P-type cation transport ATPases, and to the subfamily of aminophospholipid-transporting ATPases. The aminophospholipid translocases transport phosphatidylserine and phosphatidylethanolamine from one side of a bilayer to another. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Protein type: Hydrolase; Membrane protein, multi-pass; EC 3.6.3.1; Transporter, ion channel; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: Golgi apparatus; integral to membrane; plasma membrane

Molecular Function: phospholipid-translocating ATPase activity; magnesium ion binding; ATP binding

Biological Process: phospholipid translocation; metabolic process; transmembrane transport; Golgi organization and biogenesis