Rabbit anti-Human NDRG4 Polyclonal Antibody | anti-NDRG4 antibody

NDRG4, ID (NDRG4, BDM1, KIAA1180, Protein NDRG4, Brain development-related molecule 1, N-myc downstream-regulated gene 4 protein, Vascular smooth muscle cell-associated protein 8) (PE)

Cat. Num. AAA6324286

• Gene Names: NDRG4; BDM1; SMAP8; SMAP-8
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NDRG4; Polyclonal Antibody; ID (NDRG4; BDM1; KIAA1180; Protein NDRG4; Brain development-related molecule 1; N-myc downstream-regulated gene 4 protein; Vascular smooth muscle cell-associated protein 8) (PE); Vascular smooth muscle cell-associated protein 8; anti-NDRG4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-NDRG4 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NDRG4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-220 amino acids from the Central region of human NDRG4.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NDRG4 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NDRG4 antibody detected the NDRG4 protein (arrow).)

Related Product Information for anti-NDRG4 antibody

This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that may be involved in the regulation of mitogenic signalling in vascular smooth muscles cells. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq].

Product Categories/Family for anti-NDRG4 antibody

Antibodies; KIAA; orf

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Holm, H., et al. Nat. Genet. 42(2):117-122(2010). Schilling, S.H., et al. J. Biol. Chem. 284(37):25160-25169(2009). Melotte, V., et al. J. Natl. Cancer Inst. 101(13):916-927(2009). Pfeufer, A., et al. Nat. Genet. 41(4):407-414(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 194440722
• NCBI GeneID: 65009
• NCBI Accession #: NP_001123959.1; NP_065198.1
• NCBI GenBank Nucleotide #: NM_001130487.1
• UniProt Accession #: Q9ULP0; Q6ZNE7; Q6ZTI7; Q96PL9; Q9GZM1; Q9GZN3; Q9GZX0; B3KNU2; B4DK66; B4DSW5; H7C600
• Molecular Weight: 38,459 Da
• NCBI Official Full Name: protein NDRG4 isoform 2
• NCBI Official Synonym Full Names: NDRG family member 4
• NCBI Official Symbol: NDRG4
• NCBI Official Synonym Symbols: BDM1; SMAP8; SMAP-8
• NCBI Protein Information: protein NDRG4; smooth muscle-associated protein 8; brain development-related molecule 1; N-myc downstream-regulated gene 4 protein; vascular smooth muscle cell-associated protein 8
• UniProt Protein Name: Protein NDRG4
• Protein Family: Protein
• UniProt Gene Name: NDRG4
• UniProt Synonym Gene Names: BDM1; KIAA1180; SMAP-8
• UniProt Entry Name: NDRG4_HUMAN

NCBI Description

This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that is required for cell cycle progression and survival in primary astrocytes and may be involved in the regulation of mitogenic signalling in vascular smooth muscles cells. Alternative splicing results in multiple transcripts encoding different isoforms.[provided by RefSeq, Jun 2011]

Uniprot Description

NDRG4: Contributes to the maintenance of intracerebral BDNF levels within the normal range, which is necessary for the preservation of spatial learning and the resistance to neuronal cell death caused by ischemic stress. May enhance growth factor-induced ERK1 and ERK2 phosphorylation, including that induced by PDGF and FGF. May attenuate NGF-promoted ELK1 phosphorylation in a microtubule-dependent manner. Expressed predominantly in brain and heart. In the brain, detected in astrocytes. Isoform 1 and isoform 2 are only expressed in brain. Isoform 3 is expressed in both heart and brain. Up-regulated in glioblastoma multiforme cells. Belongs to the NDRG family. 6 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: endoplasmic reticulum membrane; mitochondrion; basolateral plasma membrane; cytoplasm; cytosol

Biological Process: negative regulation of smooth muscle cell proliferation; response to stress; embryonic heart tube development; cardiac muscle cell proliferation; heart looping; negative regulation of smooth muscle cell migration; cell growth; cell differentiation