Mouse anti-Human CXCR3 Monoclonal Antibody | anti-CXCR3 antibody

CXCR3 (CD183) (Carboxyfluorescein)

Cat. Num. AAA601531

• Gene Names: CXCR3; GPR9; MigR; CD182; CD183; Mig-R; CKR-L2; CMKAR3; IP10-R
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: CXCR3; Monoclonal Antibody; CXCR3 (CD183) (Carboxyfluorescein); Anti -CXCR3 (CD183) (Carboxyfluorescein); anti-CXCR3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2Ar1
• Specificity: Recognizes human CXCR3. Reacts specifically with CXCR3 transfectants and not the parental cell line using FACS analysis. Does not crossreact with CXCR1, CXCR2 and CXCR4 transfectants.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.1% sodium azide. Labeled with Carboxyfluorescein.
• Concentration: 0.05 mg/ml (varies by lot)

• Applicable Applications for anti-CXCR3 antibody: Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry.; Dilution: Flow Cytometry: 10ul/10e5 cells to detect human CXCR3.
• Immunogen: NSO mouse myeloma cell line transfected with human CXCR3 (aa1-368). (P49682)
• Hybridoma: Balb/c mouse inoculated with hCXCR3 transfected NS0 mouse myeloma cells.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Flow Cytometry (FC/FACS)

(Flow Cytometry detection of CXCR3 in human Lymphocytes using MBS601531.)

Related Product Information for anti-CXCR3 antibody

CXCR3 is a cell surface chemokine receptor that belongs to the family of G protein linked, seven transmembrane domain containing receptors. With the cloning of this molecule, it was identified as the specific receptor for two CXC chemokines: gamma interferon induced protein and monokine induced by interferon gamma (1). The CXCR3 receptors appear to have affinity for CXC chemokines that do not contain the conserved ELR motif at their amino terminus and as a result IL-8 and platelet factor 4 (PF4) do not bind to this receptor (2). CXCR3 shares approximately 40% amino acid homology with the CXCR1 and CXCR2 receptors, and approximately 35% homology with the first five CCR receptors (1). Even though the two ligands for CXCR3 belong to the alpha chemokine family, IP-10 and MIG are the first chemokines to target lymphocytes (1-3). CXCR3 molecules were also found by mRNA expression on activated T cells, B cells and NK cells, but not on monocytes, resting T cells or neutrophils (1,4). Of clinical interest is the demonstration of CXCR3 expression on cells from B cell chronic lymphocytic leukemias and splenic marginal zone lymphomas (5).

Product Categories/Family for anti-CXCR3 antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 218563730
• NCBI GeneID: 2833
• NCBI Accession #: NP_001136269.1
• NCBI GenBank Nucleotide #: NM_001142797.1
• UniProt Accession #: P49682; O15185; Q7Z710; Q9P2T4; Q9P2T5
• Molecular Weight: 40,660 Da
• NCBI Official Full Name: C-X-C chemokine receptor type 3 isoform 2
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) receptor 3
• NCBI Official Symbol: CXCR3
• NCBI Official Synonym Symbols: GPR9; MigR; CD182; CD183; Mig-R; CKR-L2; CMKAR3; IP10-R
• NCBI Protein Information: C-X-C chemokine receptor type 3; CXC-R3; CXCR-3; Mig receptor; IP10 receptor; IP-10 receptor; OTTHUMP00000032170; OTTHUMP00000070257; G protein-coupled receptor 9; chemokine (C-X-C) receptor 3; interferon-inducible protein 10 receptor
• UniProt Protein Name: C-X-C chemokine receptor type 3
• Protein Family: C-X-C chemokine receptor
• UniProt Gene Name: CXCR3
• UniProt Synonym Gene Names: GPR9
• UniProt Entry Name: CXCR3_HUMAN

NCBI Description

This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms (CXCR3-B) shows high affinity binding to chemokine, CXCL4/PF4 (PMID:12782716). [provided by RefSeq]

Uniprot Description

CXCR3: Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC). Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq13

Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: C-X-C chemokine binding; chemokine receptor activity; chemokine binding; receptor activity; C-X-C chemokine receptor activity

Biological Process: positive regulation of chemotaxis; apoptosis; calcium-mediated signaling; positive regulation of cAMP metabolic process; chemotaxis; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; negative regulation of angiogenesis; positive regulation of angiogenesis; cell surface receptor linked signal transduction; positive regulation of cell proliferation; regulation of leukocyte migration; negative regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; positive regulation of release of sequestered calcium ion into cytosol; cell motility; inflammatory response; cell adhesion