Western Blot (WB)
(AT8B2 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the AT8B2 antibody detected the AT8B2 protein (arrow).)
Rabbit anti-Human AT8B2 Polyclonal Antibody | anti-AT8B2 antibody
AT8B2, NT (ATP8B2, ATPID, KIAA1137, Probable phospholipid-transporting ATPase ID, ATPase class I type 8B member 2) (PE)
Gene Names
ATP8B2; ATPID
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
AT8B2; Polyclonal Antibody; NT (ATP8B2; ATPID; KIAA1137; Probable phospholipid-transporting ATPase ID; ATPase class I type 8B member 2) (PE); ATP8B2; ATPase class I type 8B member 2; anti-AT8B2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-AT8B2 antibody
Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
AT8B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-51 amino acids from the N-terminal region of human AT8B2.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(AT8B2 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the AT8B2 antibody detected the AT8B2 protein (arrow).)
Western Blot (WB)
(AT8B2 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the AT8B2 antibody detected the AT8B2 protein (arrow).)
Flow Cytometry (FC/FACS)
(AT8B2 Antibody (N-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(AT8B2 Antibody (N-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-AT8B2 antibody
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of aminophospholipid-transporting ATPases. The aminophospholipid translocases transport phosphatidylserine and phosphatidylethanolamine from one side of a bilayer to another. Alternatively spliced transcript variants encoding different isoforms have been identified.
Product Categories/Family for anti-AT8B2 antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Harris, M.J., et al. Biochim. Biophys. Acta 1633(2):127-131(2003). Halleck, M.S., et al. Physiol. Genomics 1(3):139-150(1999).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
137,440 Da
NCBI Official Full Name
phospholipid-transporting ATPase ID isoform b
NCBI Official Synonym Full Names
ATPase, aminophospholipid transporter, class I, type 8B, member 2
NCBI Official Symbol
ATP8B2
NCBI Official Synonym Symbols
ATPID
NCBI Protein Information
phospholipid-transporting ATPase ID; ATPase, class I, type 8B, member 2; probable phospholipid-transporting ATPase ID; P4-ATPase flippase complex alpha subunit ATP8B2
UniProt Protein Name
Phospholipid-transporting ATPase ID
UniProt Gene Name
ATP8B2
UniProt Synonym Gene Names
ATPID; KIAA1137
UniProt Entry Name
AT8B2_HUMAN
Similar Products
Product Notes
The AT8B2 atp8b2 (Catalog #AAA6275786) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AT8B2, NT (ATP8B2, ATPID, KIAA1137, Probable phospholipid-transporting ATPase ID, ATPase class I type 8B member 2) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AT8B2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the AT8B2 atp8b2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AT8B2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
