ABCD4 sirna

ABCD4 siRNA (Human)

Cat. Num. AAA8216972

• Gene Names: ABCD4; P70R; P79R; ABC41; MAHCJ; PMP69; PXMP1L; EST352188
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ABCD4; ABCD4 siRNA (Human); PXMP1L; ATP-binding cassette sub-family D member 4; PMP70-related protein; P70R; Peroxisomal membrane protein 1-like; PXMP1-L; Peroxisomal membrane protein 69; PMP69; ABCD4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ABCD4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 606

• Applicable Applications for ABCD4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ABCD4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ABCD4 sirna

siRNA to inhibit ABCD4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4826958
• NCBI GeneID: 5826
• NCBI Accession #: NP_005041.1
• NCBI GenBank Nucleotide #: NM_005050.3
• UniProt Accession #: O14678; Q6IAQ0; Q96E75; A8K5L7
• Molecular Weight: 68,597 Da
• NCBI Official Full Name: ATP-binding cassette sub-family D member 4
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family D (ALD), member 4
• NCBI Official Symbol: ABCD4
• NCBI Official Synonym Symbols: P70R; P79R; ABC41; MAHCJ; PMP69; PXMP1L; EST352188
• NCBI Protein Information: ATP-binding cassette sub-family D member 4
• UniProt Protein Name: ATP-binding cassette sub-family D member 4
• Protein Family: ATP-binding cassette sub-family
• UniProt Gene Name: ABCD4
• UniProt Synonym Gene Names: PXMP1L; P70R; PXMP1-L; PMP69
• UniProt Entry Name: ABCD4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. The function of this peroxisomal membrane protein is unknown. However, it is speculated that it may function as a heterodimer for another peroxisomal ABC transporter and, therefore, may modify the adrenoleukodystrophy phenotype. It may also play a role in the process of peroxisome biogenesis. Alternative splicing results in at least two different transcript variants, one which is protein-coding and one which is probably not protein-coding. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCD4: is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. The function of this peroxisomal membrane protein is unknown. However, it is speculated that it may function as a heterodimer for another peroxisomal ABC transporter and, therefore, may modify the adrenoleukodystrophy phenotype. It may also play a role in the process of peroxisome biogenesis. Alternative splicing results in at least two different transcript variants, one which is protein-coding and one which is probably not protein-coding. [provided by RefSeq, Jul 2008]

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter, ABC family; Transporter

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: peroxisomal membrane; endoplasmic reticulum membrane; lysosomal membrane; integral to membrane; peroxisome; ATP-binding cassette (ABC) transporter complex

Molecular Function: ATPase activity, coupled to transmembrane movement of substances; ATP binding

Biological Process: vitamin metabolic process; cobalamin metabolic process; transmembrane transport; water-soluble vitamin metabolic process

Disease: Methylmalonic Aciduria And Homocystinuria, Cblj Type