Typical Testing Data/Standard Curve (for reference only)
Rat Coiled-coil domain-containing protein 80 (CCDC80) ELISA Kit | CCDC80 elisa kit
Rat Coiled-coil domain-containing protein 80 (CCDC80) ELISA Kit
Gene Names
CCDC80; URB; DRO1; SSG1; okuribin
Reactivity
Rat
Synonyms
Coiled-coil domain-containing protein 80 (CCDC80); Rat Coiled-coil domain-containing protein 80 (CCDC80) ELISA Kit; CCDC80 elisa kit
Reactivity
Rat
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CCDC80 elisa kit
Intended Uses: This CCDC80 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CCDC80. This ELISA kit for research use only!
Principle of the Assay: CCDC80 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CCDC80 antibody and an CCDC80-HRP conjugate. The assay sample and buffer are incubated together with CCDC80-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCDC80 concentration since CCDC80 from samples and CCDC80-HRP conjugate compete for the anti-CCDC80 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCDC80 from the sample, fewer sites are left to bind CCDC80-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCDC80 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CCDC80 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CCDC80 antibody and an CCDC80-HRP conjugate. The assay sample and buffer are incubated together with CCDC80-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCDC80 concentration since CCDC80 from samples and CCDC80-HRP conjugate compete for the anti-CCDC80 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCDC80 from the sample, fewer sites are left to bind CCDC80-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCDC80 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CCDC80 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,174 Da
NCBI Official Full Name
coiled-coil domain-containing protein 80
NCBI Official Synonym Full Names
coiled-coil domain containing 80
NCBI Official Symbol
CCDC80
NCBI Official Synonym Symbols
URB; DRO1; SSG1; okuribin
NCBI Protein Information
coiled-coil domain-containing protein 80; steroid sensitive gene 1; nuclear envelope protein okuribin; down-regulated by oncogenes protein 1; up-regulated in BRS-3 deficient mouse homolog
UniProt Protein Name
Coiled-coil domain-containing protein 80
Protein Family
UniProt Gene Name
CCDC80
UniProt Synonym Gene Names
DRO1; URB; HBE245
UniProt Entry Name
CCD80_HUMAN
Uniprot Description
URB: Promotes cell adhesion and matrix assembly. Belongs to the CCDC80 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Extracellular matrix; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 3q13.2
Cellular Component: basement membrane
Molecular Function: heparin binding; fibronectin binding
Biological Process: extracellular matrix organization and biogenesis
Research Articles on CCDC80
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Product Notes
The Rat CCDC80 ccdc80 (Catalog #AAA7221944) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7221944 ELISA Kit recognizes Rat CCDC80. It is sometimes possible for the material contained within the vial of "Coiled-coil domain-containing protein 80 (CCDC80), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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