Typical Testing Data/Standard Curve (for reference only)
Mouse ATP-binding cassette sub-family D member 4 (ABCD4) ELISA Kit | ABCD4 elisa kit
Mouse ATP-binding cassette sub-family D member 4 (ABCD4) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ABCD4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ABCD4 antibody and an ABCD4-HRP conjugate. The assay sample and buffer are incubated together with ABCD4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ABCD4 concentration since ABCD4 from samples and ABCD4-HRP conjugate compete for the anti-ABCD4 antibody binding site. Since the number of sites is limited, as more sites are occupied by ABCD4 from the sample, fewer sites are left to bind ABCD4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ABCD4 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. The function of this peroxisomal membrane protein is unknown. However, it is speculated that it may function as a heterodimer for another peroxisomal ABC transporter and, therefore, may modify the adrenoleukodystrophy phenotype. It may also play a role in the process of peroxisome biogenesis. Alternative splicing results in at least two different transcript variants, one which is protein-coding and one which is probably not protein-coding. [provided by RefSeq, Jul 2008]
Uniprot Description
ABCD4: is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. The function of this peroxisomal membrane protein is unknown. However, it is speculated that it may function as a heterodimer for another peroxisomal ABC transporter and, therefore, may modify the adrenoleukodystrophy phenotype. It may also play a role in the process of peroxisome biogenesis. Alternative splicing results in at least two different transcript variants, one which is protein-coding and one which is probably not protein-coding. [provided by RefSeq, Jul 2008]
Protein type: Transporter; Membrane protein, integral; Membrane protein, multi-pass; Transporter, ABC family
Chromosomal Location of Human Ortholog: 14q24.3
Cellular Component: peroxisomal membrane; endoplasmic reticulum membrane; lysosomal membrane; integral to membrane; peroxisome; ATP-binding cassette (ABC) transporter complex
Molecular Function: ATPase activity, coupled to transmembrane movement of substances; ATP binding
Biological Process: vitamin metabolic process; cobalamin metabolic process; transmembrane transport; water-soluble vitamin metabolic process
Disease: Methylmalonic Aciduria And Homocystinuria, Cblj Type
Research Articles on ABCD4
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Product Notes
The Mouse ABCD4 abcd4 (Catalog #AAA7249518) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7249518 ELISA Kit recognizes Mouse ABCD4. It is sometimes possible for the material contained within the vial of "ATP-binding cassette sub-family D member 4 (ABCD4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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