Western Blot (WB)
(Western blot analysis of EFCAB4B in mouse kidney tissue lysate with EFCAB4B antibody at 1 μg/mL.)
Rabbit anti-Human, Mouse EFCAB4B Polyclonal Antibody | anti-EFCAB4B antibody
EFCAB4B Antibody
Gene Names
CRACR2A; EFCAB4B
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
EFCAB4B Antibody is affinity chromatography purified via peptide column.
Synonyms
EFCAB4B; Polyclonal Antibody; EFCAB4B Antibody; CRACR2A; EF-hand calcium-binding domain-containing protein 4B; Calcium release-activated calcium channel regulator 2A; CRAC channel regulator 2A; EF-hand calcium binding domain 4B; anti-EFCAB4B antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
EFCAB4B Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
395
Applicable Applications for anti-EFCAB4B antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
EFCAB4B antibody can be used for detection of EFCAB4B by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
EFCAB4B antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human EFCAB4B.
Buffer
EFCAB4B Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
EFCAB4B antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of EFCAB4B in mouse kidney tissue lysate with EFCAB4B antibody at 1 μg/mL.)
Western Blot (WB)
(Western blot analysis of EFCAB4B in mouse kidney tissue lysate with EFCAB4B antibody at 1 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of EFCAB4B in human kidney tissue with EFCAB4B antibody at 10 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of EFCAB4B in human kidney tissue with EFCAB4B antibody at 10 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of EFCAB4B in human kidney tissue with EFCAB4B antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of EFCAB4B in human kidney tissue with EFCAB4B antibody at 20 μg/mL.)
Related Product Information for anti-EFCAB4B antibody
EFCAB4B Antibody: EFCAB4B, also known as Calcium release-activated calcium channel regulator 2A, is a novel Ca2+-binding EF-hand protein that is thought to play a key role in store-operated Ca2+ entry in T-cells by regulating CRAC channel activation. EFCAB4B acts as a cytoplasmic calcium-sensor that forms a complex with ORAI1 and STIM1 at the junctional regions between the plasma membrane and the endoplasmic reticulum upon low Ca2+ concentration. A closely related protein, EFCAB4A, is likely to play a similar role as EFCAB4B, but the detailed function of EFCAB4A is still under investigation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
83,193 Da
NCBI Official Full Name
EF-hand calcium-binding domain-containing protein 4B isoform c
NCBI Official Synonym Full Names
calcium release activated channel regulator 2A
NCBI Official Symbol
CRACR2A
NCBI Official Synonym Symbols
EFCAB4B
NCBI Protein Information
EF-hand calcium-binding domain-containing protein 4B; CRAC regulator 2A; CRAC channel regulator 2A; EF-hand calcium binding domain 4B; calcium release-activated channel regulator 2A; calcium release-activated calcium channel regulator 2A; Ca2+ release-activated Ca2+ (CRAC) channel regulator 2A
UniProt Protein Name
EF-hand calcium-binding domain-containing protein 4B
UniProt Gene Name
CRACR2A
UniProt Synonym Gene Names
EFCAB4B; CRAC channel regulator 2A
UniProt Entry Name
EFC4B_HUMAN
Uniprot Description
EFCAB4B: Ca(2+)-binding protein that plays a key role in store- operated Ca(2+) entry (SOCE) in T-cells by regulating CRAC channel activation. Acts as a cytoplasmic calcium-sensor that facilitates the clustering of ORAI1 and STIM1 at the junctional regions between the plasma membrane and the endoplasmic reticulum upon low Ca(2+) concentration. It thereby regulates CRAC channel activation, including translocation and clustering of ORAI1 and STIM1. Upon increase of cytoplasmic Ca(2+) resulting from opening of CRAC channels, dissociates from ORAI1 and STIM1, thereby destabilizing the ORAI1-STIM1 complex. Belongs to the EFCAB4 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Calcium-binding
Chromosomal Location of Human Ortholog: 12p13.32
Cellular Component: Golgi apparatus; membrane; cytoplasm; nucleolus; nucleus
Molecular Function: protein binding; calcium ion binding
Biological Process: activation of store-operated calcium channel activity; immune system process; positive regulation of calcium ion transport
Research Articles on EFCAB4B
1. Observational study and genome-wide association study of gene-disease association. (HuGE Navigator)
Similar Products
Product Notes
The EFCAB4B cracr2a (Catalog #AAA150468) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EFCAB4B Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EFCAB4B can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). EFCAB4B antibody can be used for detection of EFCAB4B by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the EFCAB4B cracr2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EFCAB4B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
