Sushi Peptide S3 Recombinant Protein

Sushi Peptide S3, bacterial recombinant

Cat. Num. AAA845003

• Applications: SDS-Page
• Purity: >=90%
• Synonyms: Sushi Peptide S3; bacterial recombinant; Factor C Sushi 3; Sushi 3; Sushi Peptide S3 recombinant protein

• Host: E Coli
• Purity/Purification: >=90%
• Form/Format: Lyophilized from 50 mM Tris. HCl and 100 mM NaCl (pH 8.0); Appearance: Lyophilized powder

• Applicable Applications for Sushi Peptide S3 recombinant protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Reconstitute with water. Recommended conc: 0.1 - 1 mg/ml.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for Sushi Peptide S3 recombinant protein

Background: MyBioSource's Sushi Peptide S3 is a trimer of one of the high endotoxin-binding domains, Sushi 3 (or S3) within Factor C, a lipopolysaccharide (LPS)-sensitive serine protease of the horseshoe crab (Limulus Polyphemus). S3 display detergent-like properties in disrupting LPS aggregates, with specificity for palmitoyl-oleoyl-phosphatidylglycerol (POPG) resulting from electrostatic and hydrophobic forces between the peptides and the bacterial lipids. During interaction with POPG, the S3 resumes a mixture of alpha-helix and beta-sheet structures. The unsaturated nature of POPG confers fluidity and enhances insertion of the peptides into the lipid bilayer, causing maximal disruption of the bacterial membrane. In short, peptide S3 can bind to lipopolysaccharide (LPS) and inhibit the growth of Gram-negative bacteria without affecting mammalian cells. It has been shown that endotoxin activates Factor C-based catalytic coagulation cascade resulting in the gelation of Limulus blood. This process is the basis of Limulus Amebocyte Lysate (LAL) endotoxin detection method.

Product Categories/Family for Sushi Peptide S3 recombinant protein

Proteins and Enzymes; Other Proteins & EnzymesProteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & EnzymesProteins and Enzymes; Proteins and Enzymes (A-Z)