Glycine Oxidase H244K Active Protein | thiO active protein

Glycine Oxidase H244K, Bacillus subtilis recombinant

Cat. Num. AAA845245

• Applications: SDS-Page
• Purity: >=90%
• Synonyms: Glycine Oxidase H244K; Bacillus subtilis recombinant; Glycine oxidase; glycine oxygen oxidoreductase (deaminating); GO; thiO active protein

• Host: E Coli
• Purity/Purification: >=90%
• Form/Format: 4.0 mg/ml in 20 mM potassium phosphate, 100 mM NaCl, pH 8.0 and 10% glycerol ; Appearance: Liquid
• Concentration: 4.0 mg/ml (varies by lot)
• Sequence Length: 369

• Applicable Applications for thiO active protein: SDS-PAGE
• Activity (Specifications/test method): 1200 mU/mg
• Biological Activity: 1200 mU/mg
• Results: 1200 mU/mg
• Unit Definition: One unit is defined as the amount of enzyme required to convert one micromole of glycine into glyoxylate and hydrogen peroxide at pH 8.5 at 37 degree C.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for thiO active protein

Background: Glycine oxidase (GO) from Bacillus subtilis (EC 1.4.3.19) is a homotetrameric flavin-dependent oxidoreductase. Each GO monomer is non-covalently bound to flavin adenine dinucleotide. GO catalyzes oxidative deamination of various primary and secondary amines (e.g. glycine, sarcosine, N-ethylglycine) and some D-amino acids (e.g. D -alanine, D -proline, D -valine) to the corresponding alpha-keto acids and hydrogen peroxide. Primarily, glycine oxidase catalyzes the oxidation of glycine in the biosynthesis of thiamine. The variant H244K shows a higher substrate specificity ratio for glycine versus sarcosine and a 5-fold improved specific activity in comparison to the wild-type.

Product Categories/Family for thiO active protein

Proteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & EnzymesProteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 16078232
• NCBI GeneID: 939377
• NCBI Accession #: NP_389049.1
• NCBI GenBank Nucleotide #: NC_000964.3
• UniProt Accession #: O31616
• Molecular Weight: 43.1 kDa (1-369 aa, NT His Tag)
• NCBI Official Full Name: glycine oxidase
• NCBI Official Symbol: thiO
• NCBI Protein Information: glycine oxidase
• UniProt Protein Name: Glycine oxidase
• Protein Family: Glycine oxidase
• UniProt Gene Name: thiO
• UniProt Synonym Gene Names: goxB; yjbR; GO
• UniProt Entry Name: GLOX_BACSU

Uniprot Description

Catalyzes the FAD-dependent oxidative deamination of various amines and D-amino acids to yield the corresponding alpha-keto acids, ammonia/amine, and hydrogen peroxide. Oxidizes sarcosine (N-methylglycine), N-ethylglycine and glycine. Can also oxidize the herbicide glyphosate (N-phosphonomethylglycine). Displays lower activities on D-alanine, D-valine, D-proline and D-methionine. Does not act on L-amino acids and other D-amino acids. Is essential for thiamine biosynthesis since the oxidation of glycine catalyzed by ThiO generates the glycine imine intermediate (dehydroglycine) required for the biosynthesis of the thiazole ring of thiamine pyrophosphate.