SIRT2 active protein

Active SIRT2, human recombinant

Cat. Num. AAA845057

• Gene Names: SIRT2; SIR2; SIR2L; SIR2L2
• Applications: SDS-Page
• Purity: >=90%
• Synonyms: SIRT2; Active SIRT2; human recombinant; NAD-dependent protein deacetylase sirtuin-2; Regulatory protein SIR2 homolog 2; SIR2-like protein 2; SIR2L; SIR2L2.; SIRT2 active protein

• Host: E Coli
• Purity/Purification: >=90%
• Form/Format: Human Sirtuin 2 is lyophilized from 50 mM Tris, pH 8.0.; Appearance: Sirtuin 2 (SIRT2) with a His-tag is supplied as lyophilized powder.
• Sequence Length: 234

• Applicable Applications for SIRT2 active protein: SDS-PAGE; HPLC
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile dH2O to a concentration of 0.1 - 1 mg/ml and let the lyophilized pellet dissolve completely. This solution can then be diluted into other aqueous buffers and stored at 4 degree C for 1 week or -20 degree C for future use. For long term storage, it is recommended to add a carrier protein (0.1% BSA). Avoid multiple freeze/thaw cycles.
• Activity (Specifications/test method): Activity of SIRT2 was tested with MyBioSource's Sirtuin Activity assay kit, using the substrate Arg-His-Lys-Lys(Ac)-AFC, which is a fluorogenic, acetylated peptide based on residues 379-382 of p53.
• Biological Activity: >= 4 mU/mg
• Results: >= 4 mU/mg
• Unit Definition: One unit of the recombinant SIRT2 is defined as the amount of enzyme that deacetylates 1 umol of substrate Arg-His-Lys-Lys(Ac)-AFC per minute at 37 degree C in a reaction solution using Sirtuin Activity assay kit.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for SIRT2 active protein

Background: Sirtuins function as intracellular regulatory proteins. Human Sirtuin 2 is a member of the class III histone deacetylases (HDACs) and has been implicated in many cellular processes that include histone deacetylation, gene silencing, chromosomal stability, and aging. Human SIRT2 is a cytoplasmic protein responsible for the deacetylation of histone H4 and a-tubulin, a modification important for controlling the cell cycle. Specifically, SIRT2 protein co-localizes with HDAC6 and microtubules and functions as a mitotic checkpoint in preventing chromosomal instability that can lead to hyperploid cells. SIRT2 is found in many tissues, but is specifically enriched in skeletal muscle, the heart, and in oligodendroglia cells in the brain. The enzymatic activity of class III HDACs is nicotinamide adenine dinucleotide (0.1mM NAD+) dependent and insensitive to HDAC inhibitor trichostatin A.

Product Categories/Family for SIRT2 active protein

Epigenetics; Eraser Enzymes; Sirtuins (SIRTs); EnzymesProteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & EnzymesProteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 300797705
• NCBI GeneID: 22933
• NCBI Accession #: NP_001180215.1
• NCBI GenBank Nucleotide #: NM_001193286.1
• UniProt Accession #: Q8IXJ6; O95889; Q924Y7; Q9P0G8; Q9UNT0; Q9Y6E9; A8K3V1; B2RB45; U5TP13
• Molecular Weight: Human SIRT2 is a 60 kDa (2 to 389 aa + NT His Tag) protein
• NCBI Official Full Name: NAD-dependent protein deacetylase sirtuin-2 isoform 3
• NCBI Official Synonym Full Names: sirtuin 2
• NCBI Official Symbol: SIRT2
• NCBI Official Synonym Symbols: SIR2; SIR2L; SIR2L2
• NCBI Protein Information: NAD-dependent protein deacetylase sirtuin-2
• UniProt Protein Name: NAD-dependent protein deacetylase sirtuin-2
• Protein Family: NAD-dependent protein deacetylase
• UniProt Gene Name: SIRT2
• UniProt Synonym Gene Names: SIR2L; SIR2L2
• UniProt Entry Name: SIR2_HUMAN

NCBI Description

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Several transcript variants are resulted from alternative splicing of this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

SIRT2: NAD-dependent protein deacetylase, which deacetylates internal lysines on histone and non-histone proteins. Deacetylates 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Deacetylates PCK1, opposing proteasomal degradation. Deacetylates 'Lys-310' of RELA. Interacts with HDAC6, suggesting that these proteins belong to a large complex that deacetylate the cytoskeleton. Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. Inhibited by Sirtinol, A3 and M15 small molecules. Inhibited by nicotinamide. Belongs to the sirtuin family. Class I subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.1.-; Deacetylase

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: centrosome; microtubule; lateral loop; paranode region of axon; perikaryon; nuclear heterochromatin; chromosome; cytosol; centriole; chromatin silencing complex; growth cone; perinuclear region of cytoplasm; cytoplasm; plasma membrane; spindle; midbody; nucleus; myelin sheath

Molecular Function: ubiquitin binding; zinc ion binding; histone deacetylase binding; protein deacetylase activity; beta-tubulin binding; transcription factor binding; histone acetyltransferase binding; protein binding; tubulin deacetylase activity; NAD-dependent histone deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); chromatin binding; histone deacetylase activity; NAD+ ADP-ribosyltransferase activity

Biological Process: chromatin silencing at rDNA; negative regulation of autophagy; proteasomal ubiquitin-dependent protein catabolic process; phosphoinositide 3-kinase cascade; regulation of myelination; regulation of cell cycle; chromatin silencing; positive regulation of attachment of spindle microtubules to kinetochore; response to redox state; negative regulation of transcription from RNA polymerase II promoter; gene silencing; chromatin silencing at telomere; negative regulation of cell proliferation; substantia nigra development; meiotic cell cycle; protein amino acid deacetylation; cellular lipid catabolic process; protein kinase B signaling cascade; positive regulation of DNA binding; mitosis; regulation of phosphorylation; positive regulation of meiosis; transcription, DNA-dependent; regulation of exit from mitosis; hepatocyte growth factor receptor signaling pathway; histone deacetylation; negative regulation of fat cell differentiation; negative regulation of striated muscle development; myelination in the peripheral nervous system; protein amino acid ADP-ribosylation; cell division; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of cell division; autophagy; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of protein catabolic process