Nicotinamide N-Methyltransferase (hNNMT) Recombinant Protein | NNMT recombinant protein

Nicotinamide N-Methyltransferase, Human Recombinant (hNNMT)

Cat. Num. AAA844128

• Applications: SDS-Page
• Purity: >=98%
• Synonyms: Nicotinamide N-Methyltransferase (hNNMT); Nicotinamide N-Methyltransferase; Human Recombinant (hNNMT); NNMT; NNMT recombinant protein

• Host: E Coli
• Purity/Purification: >=98%
• Form/Format: 3.42 mg/ml solution in 25 mM Tris (pH8.0) buffer containing 20% glycerol; Appearance: Liquid
• Concentration: 3.42 mg/ml (varies by lot)
• Sequence Length: 264

• Applicable Applications for NNMT recombinant protein: SDS-PAGE
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -80 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for NNMT recombinant protein

Background: N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. NNMT is an important cytosolic methyltransferase catalyzing the N-methylation of nicotinamide, pyridines and structural analogs, playing a crucial role in the biotransformation and detoxification of many xenobiotic compounds. In the N-methylation process, NNMT uses S-adenosyl methionine as the methyl donor and nicotinamide as methyl acceptor. NNMT is mostly expressed in the liver, and a lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. It may also play an important role in regulating biological processes related to N-methyl nicotinamide such as anti-inflammatory, anti-thrombotic, vasoprotective, and gastroprotective properties. Increased NNMT activity was reported in many kinds of tumors. NNMT is a potential biomarker and therapeutic target in caner identification and treatment. NNMT serum levels have significance in the premature detection and in the management of patients with colorectal cancer. The recombinant protein includes hNNMT with N-terminal His-tag.

Product Categories/Family for NNMT recombinant protein

Epigenetics; Writer Enzymes; Protein Lysine Methyltransferases (PKMTs); EnzymesProteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & EnzymesProteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 5453790
• NCBI GeneID: 4837
• NCBI Accession #: NP_006160.1
• NCBI GenBank Nucleotide #: NM_006169.2
• UniProt Accession #: P40261
• Molecular Weight: 31.7 kDa (263aa + NT 6xHis-tag)
• NCBI Official Full Name: nicotinamide N-methyltransferase
• NCBI Official Synonym Full Names: nicotinamide N-methyltransferase
• NCBI Official Symbol: NNMT
• NCBI Protein Information: nicotinamide N-methyltransferase
• UniProt Protein Name: Nicotinamide N-methyltransferase
• UniProt Gene Name: NNMT
• UniProt Entry Name: NNMT_HUMAN

NCBI Description

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq, Jul 2008]

Uniprot Description

NNMT: Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. Belongs to the NNMT/PNMT/TEMT family.

Protein type: EC 2.1.1.1; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Methyltransferase

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: cytosol

Molecular Function: nicotinamide N-methyltransferase activity

Biological Process: response to drug; methylation; organ regeneration; xenobiotic metabolic process; response to organic nitrogen