Testing Data
Caspase-1 Active Protein | Casp1 active protein
Active Caspase-1, rat recombinant
Synonyms
Caspase-1; Active Caspase-1; rat recombinant; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; IL-1BC; p45.; Casp1 active protein
Host
E Coli
Form/Format
Semi-Dry
Appearance: Semi-Dry
Appearance: Semi-Dry
Sequence Length
402
Solubility/Reconstitution Instructions
Reconstitute to 1 unit per mul in PBS containing 15% glycerol.
Biological Activity
The caspase-1 is routinely tested at MyBioSource for its ability to cleave these two substrates Ac-YVAD-pNA or Ac-YVAD-AFC (1103-200).
Results
>3000 units/mg
Handling
Centrifuge the vial prior to opening.
Preparation and Storage
At -80 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Testing Data
Testing Data
Related Product Information for Casp1 active protein
Background: Caspase-1 (also known as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kDa) and small (10 kDa) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form. The recombinant rat caspase-1 was expressed in E. coli as a single polypeptide of 284 amino acids with molecular weight of 34.6. The caspase-1 is purified and activated by proprietary techniques.
Product Categories/Family for Casp1 active protein
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
~ 34.6 KDa
NCBI Official Full Name
Caspase-1
UniProt Protein Name
Caspase-1
Protein Family
UniProt Gene Name
Casp1
UniProt Synonym Gene Names
Il1bc; Il1bce; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme
UniProt Entry Name
CASP1_RAT
NCBI Description
apoptotic protease that may play a role in inflammation and apoptosis in male sex organs [RGD, Feb 2006]
Uniprot Description
CASP1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis (By similarity)
Protein type: EC 3.4.22.36; Apoptosis; Protease
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Product Notes
The Casp1 casp1 (Catalog #AAA844936) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Caspase-1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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