Testing Data
Rabbit DKK2 Polyclonal Antibody | anti-DKK2 antibody
DKK2 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity purified
Synonyms
DKK2; Polyclonal Antibody; DKK2 Antibody; DKK-2; DKK 2; dkk2; dkk-2; dkk 2; dickkopf homolog-2; dickkopf homolog2; dickkopf homolog 2; anti-DKK2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
100 ug (0.5 mg/ml) purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
Appearance: Colorless liquid
Appearance: Colorless liquid
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
259
Applicable Applications for anti-DKK2 antibody
Western Blot (WB)
Application Notes
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody recognizes 30-35 kDa Dkk2 in samples from human, mouse and rat origins. A ~25 kDa band can also be detected, presumably to be the cleavage fragment of Dkk2.
Immunogen
Synthetic peptide surrounding amino acid 241 of mouse Dkk2 (
Handling
The antibody solution should be gently mixed before use.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Testing Data
Testing Data
Related Product Information for anti-DKK2 antibody
Background: Xenopus Dickkopf (Dkk)-1 was initially discovered as a Wnt antagonist that plays an important role in head formation. By far, four members of Dkk have been identified in mammals. Each Dkk molecule contains two conserved cysteine-rich domains. Recent studies showed that the second Cys-rich domains of Dkk1 and Dkk2 inhibited Wnt-3a-activated signaling, whereas the first Cys-rich domains had no effects. In addition, the second Cys-rich domain of Dkk-2, but not that of Dkk-1, was able to activate the canonical pathway in the presence of LRP6, and this LRP-dependent signaling does not require Dvl.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,432 Da
NCBI Official Full Name
dickkopf-related protein 2
NCBI Official Synonym Full Names
dickkopf homolog 2 (Xenopus laevis)
NCBI Official Symbol
Dkk2
NCBI Protein Information
dickkopf-related protein 2
UniProt Protein Name
Dickkopf-related protein 2
Protein Family
UniProt Gene Name
Dkk2
UniProt Synonym Gene Names
Dickkopf-2; Dkk-2; mDkk-2
UniProt Entry Name
DKK2_MOUSE
Uniprot Description
DKK2: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP5 and LRP6. Expressed in heart, brain, skeletal muscle and lung. Belongs to the dickkopf family.
Protein type: Inhibitor; Secreted, signal peptide; Secreted
Cellular Component: extracellular space; extracellular region
Biological Process: negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; multicellular organismal development
Research Articles on DKK2
Similar Products
Product Notes
The DKK2 dkk2 (Catalog #AAA840830) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DKK2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DKK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody recognizes 30-35 kDa Dkk2 in samples from human, mouse and rat origins. A ~25 kDa band can also be detected, presumably to be the cleavage fragment of Dkk2. Researchers should empirically determine the suitability of the DKK2 dkk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DKK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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