Rabbit anti-Human LRRK1 Polyclonal Antibody | anti-LRRK1 antibody

LRRK1 Antibody

Cat. Num. AAA7052881

• Gene Names: LRRK1; RIPK6; Roco1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: >95%, Protein G purified
• Synonyms: LRRK1; Polyclonal Antibody; LRRK1 Antibody; Leucine-rich repeat serine/threonine-protein kinase 1; anti-LRRK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 2015

• Applicable Applications for anti-LRRK1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1:500-1:5000
• Immunogen: Recombinant Human Leucine-rich repeat serine/threonine-protein kinase 1 protein (1-68AA)
• Conjugation: Non-conjugated
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: HepG2 whole cell lysate,A549 whole cell lysateAll lanes: LRRK1 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 226,29 KDaObserved band size: 226 KDa)

Related Product Information for anti-LRRK1 antibody

cytoplasm, mitochondrion, identical protein binding, positive regulation of canonical Wnt signaling pathway

NCBI and Uniprot Product Information

• NCBI GI #: 90991702
• NCBI GeneID: 79705
• NCBI Accession #: NP_078928.3
• NCBI GenBank Nucleotide #: NM_024652.5
• UniProt Accession #: Q38SD2; Q6NVH5; Q6NYC0; Q6ZNL9; Q6ZNM9; Q96JN5; Q9H5S3
• Molecular Weight: 28,848 Da
• NCBI Official Full Name: leucine-rich repeat serine/threonine-protein kinase 1
• NCBI Official Synonym Full Names: leucine rich repeat kinase 1
• NCBI Official Symbol: LRRK1
• NCBI Official Synonym Symbols: RIPK6; Roco1
• NCBI Protein Information: leucine-rich repeat serine/threonine-protein kinase 1
• UniProt Protein Name: Leucine-rich repeat serine/threonine-protein kinase 1
• Protein Family: Leucine-rich repeat serine/threonine-protein kinase
• UniProt Gene Name: LRRK1

NCBI Description

This gene encodes a multi-domain protein that is a leucine-rich repeat kinase and a GDP/GTP binding protein. The encoded protein is thought to play a role in the regulation of bone mass. Mice lacking a similar gene showed severe osteopetrosis, increased bone mineralization and decreased bone resorption. [provided by RefSeq, Jan 2017]

Uniprot Description

LRRK1: a large multidomain protein kinase with a TKL-type kinase domain, multiple protein-protein interaction domains and a mitochondrial Rho domain (MIRO). Involved in the trafficking of epidermal growth factor (EGFR) from early to late endosomes. Forms a complex with activated EGFR via the adaptor protein Grb2. Subsequently, LRRK1 and EGFR are internalized and co-localized in early endosomes. LRRK1 is phosphorylated by EGFR, inhibiting its kinase activity. The reduction of kinase activity plays an important role in endosomal trafficking of the EGFR. Serves as a scaffold expediting the interaction of EGFR with the endosomal sorting complex required for efficient sorting of EGFR to the inner vesicles of multivesicular bodies. Plays a critical role in negative regulation of bone mass in part through modulating the c-Src signaling pathway in mice. Phosphorylation of c-Src at Tyr-527 is significantly elevated whereas at Tyr-416 is decreased in LRRK1 KO mouse osteoclasts.

Protein type: EC 2.7.11.1; Kinase, protein; LRRK family; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: cytoplasm; mitochondrion

Molecular Function: identical protein binding; protein binding; protein serine/threonine kinase activity; signal transducer activity

Biological Process: protein amino acid phosphorylation