Typical Testing Data/Standard Curve (for reference only)
Mouse Spectrin alpha chain, brain ELISA Kit | Sptan1 elisa kit
Mouse Spectrin alpha chain, brain ELISA Kit
Gene Names
Sptan1; Spna2; Spna-2; 2610027H02Rik
Reactivity
Mouse
Synonyms
Spectrin alpha chain; brain; Mouse Spectrin alpha chain; brain ELISA Kit; Alpha-II spectrin; Fodrin alpha chain; Spectrin; non-erythroid alpha chain; Sptan1; Spna2; Spta2; Sptan1 elisa kit
Reactivity
Mouse
Specificity
Recombinant and natural Mouse Spectrin alpha chain, brain
Sequence Length
2472
Samples
Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
Detection Range
0.156-10 ng/mL
Sensitivity
<0.08 ng/mL
Intra-assay Precision
Intra-Assay CV: <=6.2%
Inter-assay Precision
Inter-Assay CV: <=8.7%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Sptan1 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
282,340 Da
NCBI Official Full Name
Spectrin alpha chain, non-erythrocytic 1
NCBI Official Synonym Full Names
spectrin alpha, non-erythrocytic 1
NCBI Official Symbol
Sptan1
NCBI Official Synonym Symbols
Spna2; Spna-2; 2610027H02Rik
NCBI Protein Information
spectrin alpha chain, non-erythrocytic 1
UniProt Protein Name
Spectrin alpha chain, non-erythrocytic 1
Protein Family
UniProt Gene Name
Sptan1
UniProt Synonym Gene Names
Spna2; Spta2
UniProt Entry Name
SPTN1_MOUSE
Uniprot Description
SPTAN1: a cytoskeletal protein which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. Capable to forming dimers which can further associate to tetramers.
Protein type: Adaptor/scaffold; Cytoskeletal; Motility/polarity/chemotaxis
Cellular Component: cortical cytoskeleton; cytoplasm; cytoskeleton; fascia adherens; intracellular membrane-bound organelle; lateral plasma membrane; membrane; microtubule cytoskeleton; myelin sheath; paranode region of axon; plasma membrane; protein complex; Z disc
Molecular Function: actin binding; calcium ion binding; calmodulin binding; metal ion binding; protein binding; protein complex binding; protein heterodimerization activity; protein N-terminus binding; spectrin binding; syntaxin binding
Biological Process: actin cytoskeleton reorganization; actin filament capping
Research Articles on Sptan1
Similar Products
Product Notes
The Mouse Sptan1 sptan1 (Catalog #AAA2880422) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2880422 ELISA Kit recognizes Mouse Sptan1. It is sometimes possible for the material contained within the vial of "Spectrin alpha chain, brain, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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