Typical Standard Curve/Testing Data
Human Spectrin alpha chain ELISA Kit | SPTAN1 elisa kit
Human Spectrin alpha chain, brain ELISA Kit
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Spectrin alpha chain, brain. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forSpectrin alpha chain, brain and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Spectrin alpha chain, brain, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Spectrin alpha chain, brain in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. This gene encodes an alpha spectrin that is specifically expressed in nonerythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in this gene are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]
Uniprot Description
SPTAN1: a cytoskeletal protein which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. Capable to forming dimers which can further associate to tetramers.
Protein type: Adaptor/scaffold; Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 9q34.11
Cellular Component: cytosol; extracellular region; intracellular membrane-bound organelle; membrane; microtubule cytoskeleton; spectrin
Molecular Function: cadherin binding; protein binding; Ras guanyl-nucleotide exchange factor activity; structural constituent of cytoskeleton
Biological Process: axon guidance; ER to Golgi vesicle-mediated transport; MAPKKK cascade; neutrophil degranulation
Disease: Epileptic Encephalopathy, Early Infantile, 5
Research Articles on SPTAN1
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Product Notes
The Human SPTAN1 sptan1 (Catalog #AAA9425457) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9425457 ELISA Kit recognizes Human SPTAN1. It is sometimes possible for the material contained within the vial of "Spectrin alpha chain, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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