SPHK1 / Sphingosine Kinase 1 Recombinant Protein | SPHK1 recombinant protein

Recombinant Human SPHK1 / Sphingosine Kinase 1 Protein (His & GST tag)

Cat. Num. AAA2545017

• Gene Names: SPHK1; SPHK
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: SPHK1 / Sphingosine Kinase 1; Recombinant Human SPHK1 / Sphingosine Kinase 1 Protein (His & GST tag); SPHK1; SPHK; SPK; SPHK1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 8.0
• Sequence Length: 384

• Application Notes: The secreted recombinant human SPHK1 consists of 621 amino acids and predicts a molecular mass of 70.3 KDa. The apparent molecular mass of the protein is approximately 64 KDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for SPHK1 recombinant protein

Background: SPHK1, also known as sphingosine Kinase 1, catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P). S1P exhibits a broad spectrum of biological activities including cell proliferation, survival, migration, cytoskeletal organization, and morphogenesis. It is a ligand for cell surface G protein-coupled receptors. SPHK 1 is a potential therapeutic target for the control of cancer and inflammation. SPHK1 plays a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes.

Description: A DNA sequence encoding the human SPHK1 (Q9NYA1-1) (Met1-Leu384) was expressed the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 217272883
• NCBI GeneID: 8877
• NCBI Accession #: NP_001136073.1
• NCBI GenBank Nucleotide #: NM_001142601.1
• UniProt Accession #: Q9NYA1; Q8N632; Q96GK1; Q9HD92; Q9NY70; Q9NYL3
• Molecular Weight: 43,944 Da
• NCBI Official Full Name: sphingosine kinase 1 isoform 3
• NCBI Official Synonym Full Names: sphingosine kinase 1
• NCBI Official Symbol: SPHK1
• NCBI Official Synonym Symbols: SPHK
• NCBI Protein Information: sphingosine kinase 1
• UniProt Protein Name: Sphingosine kinase 1
• UniProt Gene Name: SPHK1
• UniProt Synonym Gene Names: SPHK; SPK; SK 1; SPK 1

NCBI Description

The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

SPHK1: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. Interacts with ACY1. Binds to calmodulin. Interacts with SPHKAP. Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.1.91; Kinase, lipid; Lipid Metabolism - sphingolipid

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: cytoplasm; cytosol; plasma membrane

Molecular Function: ATP binding; calmodulin binding; D-erythro-sphingosine kinase activity; DNA binding; magnesium ion binding; protein binding; protein phosphatase 2A binding; sphinganine kinase activity

Biological Process: activation of NF-kappaB transcription factor; calcium-mediated signaling; negative regulation of apoptosis; positive regulation of angiogenesis; positive regulation of cell growth; positive regulation of cell migration; positive regulation of fibroblast proliferation; positive regulation of mitotic cell cycle; positive regulation of NF-kappaB import into nucleus; positive regulation of protein ubiquitination; positive regulation of smooth muscle contraction; protein folding; signal transduction; sphingolipid biosynthetic process; sphingosine biosynthetic process; sphingosine metabolic process