Human SPHK1 ELISA Kit | SPHK1 elisa kit

Human SPHK1 PicoKine ELISA Kit

Cat. Num. AAA178546

• Gene Names: SPHK1; SPHK
• Reactivity: Human
• Synonyms: SPHK1; Human SPHK1 PicoKine ELISA Kit; SK 1; SK1; Sphingosine kinase 1; SPHK 1; SPK; SPK 1; Q9NYA1; SPHK1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human SPHK1
• Sequence Length: 384

• Samples: Cell culture supernates, serum and plasma(heparin, EDTA).
• Assay Type: Sandwich
• Detection Range: 312pg/ml-20000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).

Typical Testing Data/Standard Curve (for reference only)

(Human SPHK1 PicoKine ELISA Kit standard curve )

Related Product Information for SPHK1 elisa kit

Principle of the Assay: Human SPHK1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SPHK1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: M1-L384) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SPHK1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human SPHK1 amount of sample captured in plate.

Background/Introduction: Sphingosine kinase 1 is an enzyme that in humans is encoded by the SPHK1 gene. The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1- phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G proteincoupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 217272883
• NCBI GeneID: 8877
• NCBI Accession #: NP_001136073.1
• NCBI GenBank Nucleotide #: NM_001142601.1
• UniProt Accession #: Q9NYA1; Q8N632; Q96GK1; Q9HD92; Q9NY70; Q9NYL3
• Molecular Weight: 43,944 Da
• NCBI Official Full Name: sphingosine kinase 1 isoform 3
• NCBI Official Synonym Full Names: sphingosine kinase 1
• NCBI Official Symbol: SPHK1
• NCBI Official Synonym Symbols: SPHK
• NCBI Protein Information: sphingosine kinase 1
• UniProt Protein Name: Sphingosine kinase 1
• UniProt Gene Name: SPHK1
• UniProt Synonym Gene Names: SPHK; SPK; SK 1; SPK 1
• UniProt Entry Name: SPHK1_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

SPHK1: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. Interacts with ACY1. Binds to calmodulin. Interacts with SPHKAP. Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.1.91; Kinase, lipid; Lipid Metabolism - sphingolipid

Chromosomal Location of Human Ortholog: 17q25.2

Cellular Component: cytoplasm; cytosol; plasma membrane

Molecular Function: ATP binding; calmodulin binding; D-erythro-sphingosine kinase activity; DNA binding; magnesium ion binding; protein binding; protein phosphatase 2A binding; sphinganine kinase activity

Biological Process: activation of NF-kappaB transcription factor; calcium-mediated signaling; negative regulation of apoptosis; positive regulation of angiogenesis; positive regulation of cell growth; positive regulation of cell migration; positive regulation of fibroblast proliferation; positive regulation of mitotic cell cycle; positive regulation of NF-kappaB import into nucleus; positive regulation of protein ubiquitination; positive regulation of smooth muscle contraction; protein folding; signal transduction; sphingolipid biosynthetic process; sphingosine biosynthetic process; sphingosine metabolic process