Western Blot/Blotting
(Western blot analysis using Sphingosine Kinase 1 antibody at a dilution of 1:400 on MDBK cell lysate using mouse anti-rabbit HRP secondary antibody at 1:75,000.)
Rabbit Sphingosine Kinase 1 Polyclonal Antibody | anti-SPHK1 antibody
Sphingosine Kinase 1
Western Blot/Blotting
(Western blot analysis using Sphingosine Kinase 1 antibody at a dilution of 1:400 on MDBK cell lysate using mouse anti-rabbit HRP secondary antibody at 1:75,000.)
Western Blot/Blotting
(Western blot analysis using Sphingosine Kinase 1 antibody at a dilution of 1:400 on MDBK cell lysate using mouse anti-rabbit HRP secondary antibody at 1:75,000.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Uniprot Description
Function: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. Ref.12
Catalytic activity: ATP + sphinganine = ADP + sphinganine 1-phosphate. Ref.2 Ref.12ATP + sphingosine = ADP + sphingosine 1-phosphate. Ref.2 Ref.12
Cofactor: Magnesium. Ref.3
Subunit structure: Interacts with ACY1
By similarity. Binds to calmodulin. Interacts with SPHKAP. Ref.9
Subcellular location: Cytoplasm. Nucleus Ref.10.
Tissue specificity: Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. Ref.2
Miscellaneous: Sphingosine 1-phosphate stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling.
Sequence similarities: Contains 1 DAGKc domain.
Biophysicochemical propertiesKinetic parameters:KM=14 µM for sphingosine Ref.3KM=20 µM for dihydrosphingosineKM=77 µM for ATPpH dependence:Optimum pH is 7.4.
Research Articles on SPHK1
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Product Notes
The SPHK1 sphk1 (Catalog #AAA395917) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Sphingosine Kinase 1 reacts with Human, Rat, Mouse, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's Sphingosine Kinase 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Researchers should empirically determine the suitability of the SPHK1 sphk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sphingosine Kinase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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